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Gel Phase 1,2-Diste...
Gel Phase 1,2-Distearoyl-sn-glycero-3-phosphocholine-Based Liposomes Are Superior to Fluid Phase Liposomes at Augmenting Both Antigen Presentation on Major Histocompatibility Complex Class II and Costimulatory Molecule Display by Dendritic Cells in Vitro
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- Norling, Karin, 1988 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Division of Biological Physics, Department of Physics, Chalmers University of Technology, 412 96 Gothenburg, Sweden
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- Bernasconi, Valentina, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,University of Gothenburg,Mucosal Immunobiology and Vaccine Center (MIVAC), Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, 405 30 Gothenburg, Sweden
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- Agmo Hernandez, Victor (author)
- Uppsala universitet,Analytisk kemi,Institutionen för farmaci
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- Parveen, Nagma, 1988 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Katholieke Universiteit Leuven,Division of Biological Physics, Department of Physics, Chalmers University of Technology, 412 96 Gothenburg, Sweden
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- Edwards, Katarina (author)
- Uppsala universitet,Analytisk kemi
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- Lycke, Nils Y, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,University of Gothenburg,Mucosal Immunobiology and Vaccine Center (MIVAC), Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, 405 30 Gothenburg, Sweden
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- Höök, Fredrik, 1966 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Division of Biological Physics, Department of Physics, Chalmers University of Technology, 412 96 Gothenburg, Sweden
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- Bally, Marta, 1981 (author)
- Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Avdelningen för virologi,Section of Virology, Department of Clinical Microbiology, Umeå University, 901 85 Umeå, Sweden; Wallenberg Centre for Molecular Medicine, Umeå University, 901 85 Umeå, Sweden
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Parveen, Na Ma (author)
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(creator_code:org_t)
- 2019-09-09
- 2019
- English.
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In: ACS Infectious Diseases. - : American Chemical Society (ACS). - 2373-8227. ; 5:11, s. 1867-1878
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Abstract
Subject headings
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- Lipid-based nanoparticles have in recent years attracted increasing attention as pharmaceutical carriers. In particular, reports of them having inherent adjuvant properties combined with their ability to protect antigen from degradation make them suitable as vaccine vectors. However, the physicochemical profile of an ideal nanoparticle for vaccine delivery is still poorly defined. Here, we used an in vitro dendritic cell assay to assess the immunogenicity of a variety of liposome formulations as vaccine carriers and adjuvants. Using flow cytometry, we investigated liposome-assisted antigen presentation as well as the expression of relevant costimulatory molecules on the cell surface. Cytokine secretion was further evaluated with an enzyme-linked immunosorbent assay (ELISA). We show that liposomes can successfully enhance antigen presentation and maturation of dendritic cells, as compared to vaccine fusion protein (CTA1-3E alpha-DD) administered alone. In particular, the lipid phase state of the membrane was found to greatly influence the vaccine antigen processing by dendritic cells. As compared to their fluid phase counterparts, gel phase liposomes were more efficient at improving antigen presentation. They were also superior at upregulating the costimulatory molecules CD80 and CD86 as well as increasing the release of the cytokines IL-6 and IL-1 beta. Taken together, we demonstrate that gel phase liposomes, while nonimmunogenic on their own, significantly enhance the antigen-presenting ability of dendritic cells and appear to be a promising way forward to improve vaccine immunogenicity.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- lipid nanoparticle
- adjuvant effect
- dendritic cell activation
- immunogenicity
- antigen delivery
- mucosal vaccination
- mhc class-ii
- interbilayer transfer
- immune-responses
- fusion protein
- vaccine
- cd4
- ubiquitination
- cholesterol
- adjuvants
- receptor
- Pharmacology & Pharmacy
- Infectious Diseases
- lipid nanoparticle
Publication and Content Type
- ref (subject category)
- art (subject category)
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Norling, Karin, ...
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Bernasconi, Vale ...
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Agmo Hernandez, ...
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Parveen, Nagma, ...
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Edwards, Katarin ...
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Lycke, Nils Y, 1 ...
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Höök, Fredrik, 1 ...
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Bally, Marta, 19 ...
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Parveen, Na Ma
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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NATURAL SCIENCES
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and Physical Science ...
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MEDICAL AND HEAL ...
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MEDICAL AND HEAL ...
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NATURAL SCIENCES
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ACS Infectious D ...
- By the university
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University of Gothenburg
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Chalmers University of Technology
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Umeå University
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Uppsala University