Interbase FRET in RNA: from A to Z

Füchtbauer, Anders Foller, 1984 (author)

Sandberg Wranne, Moa, 1986 (author): Chalmers tekniska högskola,Chalmers University of Technology

Bood, Mattias (author): Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg,AstraZeneca AB

Weis, Erik (author): Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,AstraZeneca AB,University of Gothenburg

Pfeiffer, Pauline, 1994 (author): Chalmers tekniska högskola,Chalmers University of Technology

Nilsson, Jesper, 1984 (author): Chalmers tekniska högskola,Chalmers University of Technology

Grötli, Morten, 1966 (author): Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg

Wilhelmsson, Marcus, 1974 (author): Chalmers tekniska högskola,Chalmers University of Technology

(creator_code:org_t)

2019-09-23
2019
English.
In: Nucleic Acids Research. - : Oxford University Press (OUP). - 0305-1048 .- 1362-4962. ; 47:19, s. 9990-9997

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Abstract Subject headings

Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. We here report the first RNA base analogue FRET pair, consisting of the donor tC(O) and the non-emissive acceptor tC(nitro). The acceptor ribonucleoside is here synthesised and incorporated into RNA for the first time. This FRET pair accurately reports the average structure of A-form RNA, and its utility for probing RNA structural changes is demonstrated by monitoring the transition from A- to Z-form RNA. Finally, the measured FRET data were compared with theoretical FRET patterns obtained from two previously reported Z-RNA PDB structures, to shed new light on this elusive RNA conformation.

NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)

By the author/editor: Füchtbauer, Ande ...; Sandberg Wranne, ...; Bood, Mattias; Weis, Erik; Pfeiffer, Paulin ...; Nilsson, Jesper, ...; show more...; Dahlen, A.; Grötli, Morten, ...; Wilhelmsson, Mar ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Physical Chemist ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Structural Biolo ...

By the university: University of Gothenburg; Chalmers University of Technology

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