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Interbase FRET in R...
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Füchtbauer, Anders Foller,1984
(author)
Interbase FRET in RNA: from A to Z
- Article/chapterEnglish2019
Publisher, publication year, extent ...
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2019-09-23
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Oxford University Press (OUP),2019
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LIBRIS-ID:oai:gup.ub.gu.se/287117
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https://gup.ub.gu.se/publication/287117URI
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https://doi.org/10.1093/nar/gkz812DOI
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https://research.chalmers.se/publication/513641URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. We here report the first RNA base analogue FRET pair, consisting of the donor tC(O) and the non-emissive acceptor tC(nitro). The acceptor ribonucleoside is here synthesised and incorporated into RNA for the first time. This FRET pair accurately reports the average structure of A-form RNA, and its utility for probing RNA structural changes is demonstrated by monitoring the transition from A- to Z-form RNA. Finally, the measured FRET data were compared with theoretical FRET patterns obtained from two previously reported Z-RNA PDB structures, to shed new light on this elusive RNA conformation.
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Sandberg Wranne, Moa,1986Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)moasan
(author)
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Bood, MattiasGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg,AstraZeneca AB(Swepub:gu)xbooma
(author)
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Weis, ErikGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,AstraZeneca AB,University of Gothenburg
(author)
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Pfeiffer, Pauline,1994Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)paupfe
(author)
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Nilsson, Jesper,1984Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)jespern
(author)
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Dahlen, A.AstraZeneca AB
(author)
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Grötli, Morten,1966Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:cth)grotli
(author)
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Wilhelmsson, Marcus,1974Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)mawi
(author)
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Chalmers tekniska högskolaInstitutionen för kemi och molekylärbiologi
(creator_code:org_t)
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In:Nucleic Acids Research: Oxford University Press (OUP)47:19, s. 9990-99970305-10481362-4962
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Bood, Mattias
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Weis, Erik
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Nilsson, Jesper, ...
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Dahlen, A.
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