SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:gup.ub.gu.se/287440"
 

Search: onr:"swepub:oai:gup.ub.gu.se/287440" > The SH3 domains of ...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Andersen, T. C. B. (author)

The SH3 domains of the protein kinases ITK and LCK compete for adjacent sites on T cell?specific adapter protein

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • 2019

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/287440
  • https://gup.ub.gu.se/publication/287440URI
  • https://doi.org/10.1074/jbc.RA119.008318DOI

Supplementary language notes

  • Language:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • T-cell activation requires stimulation of specific intracellular signaling pathways in which protein-tyrosine kinases, phosphatases, and adapter proteins interact to transmit signals from the T-cell receptor to the nucleus. Interactions of LCK proto-oncogene, SRC family tyrosine kinase (LCK), and the IL-2?inducible T cell kinase (ITK) with the T cell-specific adapter protein (TSAD) promotes LCK-mediated phosphorylation and thereby ITK activation. Both ITK and LCK interact with TSAD's proline-rich region (PRR) through their Src homology 3 (SH3) domains. Whereas LCK may also interact with TSAD through its SH2 domain, ITK interacts with TSAD only through its SH3 domain. To begin to understand on a molecular level how the LCK SH3 and ITK SH3 domains interact with TSAD in human HEK293T cells, here we combined biochemical analyses with NMR spectroscopy. We found that the ITK and LCK SH3 domains potentially have adjacent and overlapping binding sites within the TSAD PRR amino acids (aa) 239?274. Pulldown experiments and NMR spectroscopy revealed that both domains may bind to TSAD aa 239?256 and aa 257?274. Co-immunoprecipitation experiments further revealed that both domains may also bind simultaneously to TSAD aa 242?268. Accordingly, NMR spectroscopy indicated that the SH3 domains may compete for these two adjacent binding sites. We propose that once the associations of ITK and LCK with TSAD promote the ITK and LCK interaction, the interactions among TSAD, ITK, and LCK are dynamically altered by ITK phosphorylation status.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Kristiansen, P. E. (author)
  • Huszenicza, Z. (author)
  • Johansson, Maria U,1971Gothenburg University,Göteborgs universitet,Svenskt NMR-centrum vid Göteborgs universitet,Swedish NMR Centre at Göteborg University(Swepub:gu)xjmard (author)
  • Gopalakrishnan, R. P. (author)
  • Kjelstrup, H. (author)
  • Boyken, S. (author)
  • Sundvold-Gjerstad, V. (author)
  • Granum, S. (author)
  • Sorlie, M. (author)
  • Backe, P. H. (author)
  • Fulton, D. B. (author)
  • Karlsson, B Göran,1962Gothenburg University,Göteborgs universitet,Svenskt NMR-centrum vid Göteborgs universitet,Swedish NMR Centre at Göteborg University(Swepub:gu)xkargo (author)
  • Andreotti, A. H. (author)
  • Spurkland, A. (author)
  • Göteborgs universitetSvenskt NMR-centrum vid Göteborgs universitet (creator_code:org_t)

Related titles

  • In:Journal of Biological Chemistry294:42, s. 15480-154940021-9258

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view