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Cathelicidin Contri...
Cathelicidin Contributes to the Restriction of Leishmania in Human Host Macrophages
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Crauwels, P. (author)
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Bank, E. (author)
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Walber, B. (author)
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- Wenzel, Ulf Alexander, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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- Agerberth, B. (author)
- Karolinska Institutet
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Chanyalew, M. (author)
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Abebe, M. (author)
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Konig, R. (author)
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Ritter, U. (author)
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Reiling, N. (author)
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van Zandbergen, G. (author)
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(creator_code:org_t)
- 2019-11-22
- 2019
- English.
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In: Frontiers in Immunology. - : Frontiers Media SA. - 1664-3224. ; 10
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Abstract
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- In cutaneous Leishmaniasis the parasitic control in human host macrophages is still poorly understood. We found an increased expression of the human cathelicidin CAMP in skin lesions of Ethiopian patients with cutaneous leishmaniasis. Vitamin D driven, Cathelicidin-type antimicrobial peptides (CAMP) play an important role in the elimination of invading microorganisms. Recombinant cathelicidin was able to induce cell-death characteristics in Leishmania in a dose dependent manner. Using human primary macrophages, we demonstrated pro-inflammatory macrophages (hMDM1) to express a higher level of human cathelicidin, both on gene and protein level, compared to anti-inflammatory macrophages (hMDM2). Activating the CAMP pathway using Vitamin D in hMDM1 resulted in a cathelicidin-mediated-Leishmania restriction. Finally, a reduction of cathelicidin in hMDM1, using a RNA interference (RNAi) approach, increased Leishmania parasite survival. In all, these data show the human cathelicidin to contribute to the innate immune response against Leishmaniasis in a human primary cell model.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Leishmania
- human macrophages
- vitamin D
- cathelicidin (LL-37)
- human
- primary immune cells
- antimicrobial activity
- vitamin-d-receptor
- nitric-oxide
- cutting edge
- antimicrobial peptides
- immune-response
- camp gene
- ll-37
- infection
- skin
- induction
- Immunology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Crauwels, P.
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Bank, E.
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Walber, B.
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Wenzel, Ulf Alex ...
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Agerberth, B.
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Chanyalew, M.
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show more...
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Abebe, M.
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Konig, R.
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Ritter, U.
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Reiling, N.
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van Zandbergen, ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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Frontiers in Imm ...
- By the university
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University of Gothenburg
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Karolinska Institutet