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Human cerebrospinal...
Human cerebrospinal fluid 6E10-immunoreactive protein species contain amyloid precursor protein fragments.
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Grant, Marianne K O (author)
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Handoko, Maureen (author)
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- Rozga, Malgorzata, 1980 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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- Brinkmalm, Gunnar (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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- Portelius, Erik, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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- Blennow, Kaj, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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Ashe, Karen H (author)
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Zahs, Kathleen R (author)
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Liu, Peng (author)
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(creator_code:org_t)
- 2019-02-28
- 2019
- English.
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In: PloS one. - : Public Library of Science (PLoS). - 1932-6203. ; 14:2
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Abstract
Subject headings
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- In a previous study, we reported that levels of two types of protein species-a type of ~55-kDa species and a type of ~15-kDa species-are elevated in the lumbar cerebrospinal fluid (CSF) of cognitively intact elderly individuals who are at risk for Alzheimer's disease (AD). These species are immunoreactive to the monoclonal antibody 6E10, which is directed against amino acids 6-10 of amyloid-β (Aβ), and their levels correlate with levels of total tau and tau phosphorylated at Thr181. In this study, we investigated the molecular composition of these AD-related proteins using immunoprecipitation (IP)/Western blotting coupled with IP/mass spectrometry. We show that canonical Aβ1-40/42 peptides, together with amyloid-β precursor protein (APP) fragments located N-terminally of Aβ, are present in the ~55-kDa, 6E10-immunoreactive species. We demonstrate that APP fragments located N-terminally of Aβ, plus the N-terminal region of Aβ, are present in the ~15-kDa, 6E10-immunoreactive species. These findings add to the catalog of AD-related Aβ/APP species found in CSF and should motivate further study to determine whether these species may serve as biomarkers of disease progression.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Aged
- Aged
- 80 and over
- Alzheimer Disease
- cerebrospinal fluid
- diagnosis
- Amino Acid Sequence
- Amyloid beta-Peptides
- cerebrospinal fluid
- chemistry
- immunology
- Biomarkers
- cerebrospinal fluid
- Blotting
- Western
- Disease Progression
- Female
- Humans
- Immunoprecipitation
- Male
- Mass Spectrometry
- Middle Aged
- Molecular Weight
- Peptide Fragments
- cerebrospinal fluid
- chemistry
- immunology
Publication and Content Type
- ref (subject category)
- art (subject category)
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PloS one
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To the university's database
- By the author/editor
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Grant, Marianne ...
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Handoko, Maureen
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Rozga, Malgorzat ...
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Brinkmalm, Gunna ...
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Portelius, Erik, ...
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Blennow, Kaj, 19 ...
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show more...
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Ashe, Karen H
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Zahs, Kathleen R
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Liu, Peng
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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PloS one
- By the university
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University of Gothenburg