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Continuous mitotic ...
Continuous mitotic activity of primitive hematopoietic stem cells in adult mice
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- Morcos, M. N. F. (author)
- Dresden University of Technology
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- Zerjatke, T. (author)
- Dresden University of Technology
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- Glauche, I. (author)
- Dresden University of Technology
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- Munz, C. M. (author)
- Dresden University of Technology
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- Ge, Y. (author)
- Dresden University of Technology
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- Petzold, A. (author)
- Dresden University of Technology
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- Reinhardt, S. (author)
- Dresden University of Technology
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- Dahl, A. (author)
- Dresden University of Technology
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- Anstee, N. S. (author)
- German Cancer Research Centre
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- Bogeska, R. (author)
- German Cancer Research Centre
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- Milsom, M. D. (author)
- German Cancer Research Centre
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- Säwén, Petter (author)
- Lund University,Lunds universitet,Avdelningen för molekylär hematologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Utvecklingshematopoes,Forskargrupper vid Lunds universitet,Division of Molecular Hematology (DMH),Department of Laboratory Medicine,Faculty of Medicine,Developmental Hematopoiesis,Lund University Research Groups
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- Wan, Haixia (author)
- Lund University,Lunds universitet,Avdelningen för molekylär hematologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Utvecklingshematopoes,Forskargrupper vid Lunds universitet,Division of Molecular Hematology (DMH),Department of Laboratory Medicine,Faculty of Medicine,Developmental Hematopoiesis,Lund University Research Groups
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- Bryder, David (author)
- University of Gothenburg,Lund University,Lunds universitet,Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center,Avdelningen för molekylär hematologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Utvecklingshematopoes,Forskargrupper vid Lunds universitet,Division of Molecular Hematology (DMH),Department of Laboratory Medicine,Faculty of Medicine,Developmental Hematopoiesis,Lund University Research Groups
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- Roers, A. (author)
- Dresden University of Technology
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- Gerbaulet, A. (author)
- Dresden University of Technology
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(creator_code:org_t)
- 2020-04-16
- 2020
- English.
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In: Journal of Experimental Medicine. - : Rockefeller University Press. - 0022-1007 .- 1540-9538. ; 217:6
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Abstract
Subject headings
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- The proliferative activity of aging hematopoietic stem cells (HSCs) is controversially discussed. Inducible fluorescent histone 2B fusion protein (H2B-FP) transgenic mice are important tools for tracking the mitotic history of murine HSCs in label dilution experiments. A recent study proposed that primitive HSCs symmetrically divide only four times to then enter permanent quiescence. We observed that background fluorescence due to leaky H2B-FP expression, occurring in all H2B-FP transgenes independent of label induction, accumulated with age in HSCs with high repopulation potential. We argue that this background had been misinterpreted as stable retention of induced label. We found cell division-independent half-lives of H2B-FPs to be short, which had led to overestimation of HSC divisional activity. Our data do not support abrupt entry of HSCs into permanent quiescence or sudden loss of regeneration potential after four divisions, but show that primitive HSCs of adult mice continue to cycle rarely.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Keyword
- self-renewal
- gene-expression
- dynamics
- exchange
- proliferation
- heterogeneity
- history
- cd34
- h3
- Immunology
- Research & Experimental Medicine
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Morcos, M. N. F.
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Zerjatke, T.
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Glauche, I.
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Munz, C. M.
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Ge, Y.
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Petzold, A.
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show more...
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Reinhardt, S.
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Dahl, A.
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Anstee, N. S.
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Bogeska, R.
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Milsom, M. D.
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Säwén, Petter
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Wan, Haixia
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Bryder, David
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Roers, A.
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Gerbaulet, A.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Hematology
- Articles in the publication
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Journal of Exper ...
- By the university
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University of Gothenburg
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Lund University