Ultrasensitive and Selective Copper(II) Detection: Introducing a Bioinspired and Robust Sensor

A nanopore-based Cu-II-sensing system is reported that allows for an ultrasensitive and selective detection of Cu(II)with the possibility for a broad range of applications, for example in medical diagnostics. A fluorescent ATCUN-like peptide 5/6-FAM-Dap-beta-Ala-His is employed to selectively bind Cu(II)ions in the presence of Ni(II)and Zn(II)and was crafted into ion track-etched nanopores. Upon Cu(II)binding the fluorescence of the peptide sensor is quenched, permitting the detection of Cu(II)in solution. The ion transport characteristics of peptide-modified nanopore are shown to be extremely sensitive and selective towards Cu(II)allowing to sense femtomolar Cu(II)concentrations in human urine mimics. Washing with EDTA fully restores the Cu-II-binding properties of the sensor, enabling multiple repetitive measurements. The robustness of the system clearly has the potential to be further developed into an easy-to-use, lab-on-chip Cu-II-sensing device, which will be of great importance for bedside diagnosis and monitor of Cu(II)levels in patients with copper-dysfunctional homeostasis.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Neurovetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Neurosciences hsv//eng
ATCUN
copper
fluorescence
nanopores
sensors
metal-binding
alzheimers-disease
nanopore sensor
atcun motif
cu(ii)
serum
ions
peptides
design
Chemistry

Duznovic, I. (author)
Tietze, Daniel,1980Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xtieda (author)
Weber, W. (author)
Ali, M. (author)
Stein, V. (author)
Ensinger, W. (author)
Tietze, Alesia A.,1984Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xtieal (author)
Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

By the author/editor: Muller, L. K.; Duznovic, I.; Tietze, Daniel, ...; Weber, W.; Ali, M.; Stein, V.; show more...; Ensinger, W.; Tietze, Alesia A ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Neurosciences

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