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Long-term in vivo i...
Long-term in vivo integrity and safety of3D-bioprinted cartilaginous constructs
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- Apelgren, Peter (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,University of Gothenburg
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- Amoroso, Matteo, 1984 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,University of Gothenburg
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- Säljö, Karin, 1981 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,University of Gothenburg
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- Lindahl, A. (author)
- Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital
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- Brantsing, C. (author)
- Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital
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- Strid Orrhult, Linnea, 1983 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Markstedt, Kajsa, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Gatenholm, Paul, 1956 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Kölby, Lars, 1963 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,University of Gothenburg
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(creator_code:org_t)
- 2020-07-06
- 2021
- English.
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In: Journal of Biomedical Materials Research Part B-Applied Biomaterials. - : Wiley. - 1552-4973 .- 1552-4981. ; 109:1, s. 126-136
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Abstract
Subject headings
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- Long-term stability and biological safety are crucial for translation of 3D-bioprinting technology into clinical applications. Here, we addressed the long-term safety and stability issues associated with 3D-bioprinted constructs comprising a cellulose scaffold and human cells (chondrocytes and stem cells) over a period of 10 months in nude mice. Our findings showed that increasing unconfined compression strength over time significantly improved the mechanical stability of the cell-containing constructs relative to cell-free scaffolds. Additionally, the cell-free constructs exhibited a mean compressive stress and stiffness (compressive modulus) of 0.04 +/- 0.05 MPa and 0.14 +/- 0.18 MPa, respectively, whereas these values for the cell-containing constructs were 0.11 +/- 0.08 MPa (p= .019) and 0.53 +/- 0.59 MPa (p= .012), respectively. Moreover, histomorphologic analysis revealed that cartilage formed from the cell-containing constructs harbored an abundance of proliferating chondrocytes in clusters, and after 10 months, resembled native cartilage. Furthermore, extension of the experiment over the complete lifecycle of the animal model revealed no signs of ossification, fibrosis, necrosis, or implant-related tumor development in the 3D-bioprinted constructs. These findings confirm the in vivo biological safety and mechanical stability of 3D-bioprinted cartilaginous tissues and support their potential translation into clinical applications.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Keyword
- 3D-bioprinting
- cartilage
- chondrocytes
- in vivo
- long-term
- nude mice
- mesenchymal stem-cells
- balb/c-nu mice
- chondrogenic differentiation
- regenerative medicine
- spontaneous tumors
- life-span
- chondrocytes
- reconstruction
- proliferation
- nanocellulose
- Engineering
- Materials Science
- chondrocytes
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Apelgren, Peter
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Amoroso, Matteo, ...
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Säljö, Karin, 19 ...
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Lindahl, A.
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Brantsing, C.
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Strid Orrhult, L ...
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show more...
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Markstedt, Kajsa ...
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Gatenholm, Paul, ...
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Kölby, Lars, 196 ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Surgery
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomaterials Sci ...
- Articles in the publication
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Journal of Biome ...
- By the university
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University of Gothenburg
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Chalmers University of Technology