Long-term in vivo integrity and safety of3D-bioprinted cartilaginous constructs

Apelgren, PeterGothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,University of Gothenburg (author)

Long-term stability and biological safety are crucial for translation of 3D-bioprinting technology into clinical applications. Here, we addressed the long-term safety and stability issues associated with 3D-bioprinted constructs comprising a cellulose scaffold and human cells (chondrocytes and stem cells) over a period of 10 months in nude mice. Our findings showed that increasing unconfined compression strength over time significantly improved the mechanical stability of the cell-containing constructs relative to cell-free scaffolds. Additionally, the cell-free constructs exhibited a mean compressive stress and stiffness (compressive modulus) of 0.04 +/- 0.05 MPa and 0.14 +/- 0.18 MPa, respectively, whereas these values for the cell-containing constructs were 0.11 +/- 0.08 MPa (p= .019) and 0.53 +/- 0.59 MPa (p= .012), respectively. Moreover, histomorphologic analysis revealed that cartilage formed from the cell-containing constructs harbored an abundance of proliferating chondrocytes in clusters, and after 10 months, resembled native cartilage. Furthermore, extension of the experiment over the complete lifecycle of the animal model revealed no signs of ossification, fibrosis, necrosis, or implant-related tumor development in the 3D-bioprinted constructs. These findings confirm the in vivo biological safety and mechanical stability of 3D-bioprinted cartilaginous tissues and support their potential translation into clinical applications.

Amoroso, Matteo,1984Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,University of Gothenburg(Swepub:gu)xamoma (author)
Säljö, Karin,1981Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,University of Gothenburg(Swepub:gu)xsaljk (author)
Lindahl, A.Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital (author)
Brantsing, C.Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital (author)
Strid Orrhult, Linnea,1983Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)lstrid (author)
Markstedt, Kajsa,1989Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)kajsam (author)
Gatenholm, Paul,1956Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)gatenh (author)
Kölby, Lars,1963Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,University of Gothenburg(Swepub:gu)xkolbl (author)
Göteborgs universitetInstitutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi (creator_code:org_t)

In:Journal of Biomedical Materials Research Part B-Applied Biomaterials: Wiley109:1, s. 126-1361552-49731552-4981

By the author/editor: Apelgren, Peter; Amoroso, Matteo, ...; Säljö, Karin, 19 ...; Lindahl, A.; Brantsing, C.; Strid Orrhult, L ...; show more...; Markstedt, Kajsa ...; Gatenholm, Paul, ...; Kölby, Lars, 196 ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Surgery

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Cell Biology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Biomaterials Sci ...

By the university: University of Gothenburg; Chalmers University of Technology

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