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Intracellular pH re...
Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas
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- Ramesh, Kirti (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences
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Hu, M. Y. (author)
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Melzner, F. (author)
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Bleich, M. (author)
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Himmerkus, N. (author)
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(creator_code:org_t)
- 2020-08-20
- 2020
- English.
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In: Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology. - : Springer Science and Business Media LLC. - 0174-1578. ; 190, s. 691-700
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https://doi.org/10.1...
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Abstract
Subject headings
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- Shell formation and repair occurs under the control of mantle epithelial cells in bivalve molluscs. However, limited information is available on the precise acid-base regulatory machinery present within these cells, which are fundamental to calcification. Here, we isolate mantle epithelial cells from the Pacific oyster,Crassostrea gigasand utilise live cell imaging in combination with the fluorescent dye, BCECF-AM to study intracellular pH (pH(i)) regulation. To elucidate the involvement of various ion transport mechanisms, modified seawater solutions (low sodium, low bicarbonate) and specific inhibitors for acid-base proteins were used. Diminished pH recovery in the absence of Na(+)and under inhibition of sodium/hydrogen exchangers (NHEs) implicate the involvement of a sodium dependent cellular proton extrusion mechanism. In addition, pH recovery was reduced under inhibition of carbonic anhydrases. These data provide the foundation for a better understanding of acid-base regulation underlying the physiology of calcification in bivalves.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Mollusc
- Calcification
- Acid-base
- Ion transport proteins
- oyster crassostrea-gigas
- carbonic-anhydrase activity
- acid-base status
- ocean acidification
- energy-metabolism
- na+/h+-exchanger
- mollusk
- biomineralization
- exposure
- calcification
- Physiology
- Zoology
Publication and Content Type
- ref (subject category)
- art (subject category)
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