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Use of the cell div...
Use of the cell division assay to diagnose Fanconi anemia patients' hypersensitivity to mitomycin C
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- Hammarsten, Ola (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine
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Muslimovic, A. (author)
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- Thunström, Sofia (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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Ek, T. (author)
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Johansson, P. (author)
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(creator_code:org_t)
- 2020-08-28
- 2021
- English.
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In: Cytometry Part B-Clinical Cytometry. - : Wiley. - 1552-4949 .- 1552-4957. ; 100:3, s. 1894-1906
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Abstract
Subject headings
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- The recently reported cell division assay (CDA) was optimized to measure the relative sensitivity of cells to cytotoxic drugs in vitro. Here, we investigated the in vitro hypersensitivity of lymphocytes from Fanconi anemia (FA) patients, to cytotoxic drugs using CDA. Peripheral blood mononuclear cells (PBMC) as well as cell lines derived from FA patients were treated with two DNA interstrand crosslinking (ICL) agents, mitomycin C and cyclophosphamide. Our data indicate that the CDA detects hypersensitivity of cells from FA patients to mitomycin C. Further, cell lines derived from FA-patients were also hypersensitive to mitomycin C as well as cyclophosphamide, when assayed by the CDA. This study suggests that the CDA is a useful alternative for the diagnosis of FA patients' hypersensitivity to ICL agents.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- cell division assay (CDA)
- DNA interstrand crosslinking (ICL) agents
- Fanconi anemia (FA)
- flow cytometry
- mitomycin C
- bone-marrow-transplantation
- fanconi-anemia
- diagnosis
- sensitivity
- telangiectasia
- radiation
- cancer
- repair
- Medical Laboratory Technology
- Pathology
Publication and Content Type
- ref (subject category)
- art (subject category)
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