Stress impairs skin barrier function and induces α2-3 linked n-acetylneuraminic acid and core 1 o-glycans on skin mucins in atlantic salmon, salmo salar

Benktander, John (author): Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology

Sundh, Henrik, 1976 (author): Gothenburg University,Göteborgs universitet,SWEMARC,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences

Sundell, Kristina, 1959 (author): Gothenburg University,Göteborgs universitet,SWEMARC,Institutionen för biologi och miljövetenskap, Kristineberg,Department of Biological and Environmental Sciences, Kristineberg

Murugan, Abarna V M (author): Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology

Venkatakrishnan, Vignesh, 1987 (author): Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology

Padra, János T (author): Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology

Kolarevic, Jelena (author)

Terjesen, Bendik Fyhn (author)

Gorissen, Marnix (author)

Lindén, Sara K., 1974 (author): Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology

(creator_code:org_t)

2021-02-02
2021
English.
In: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 22:3

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Abstract Subject headings

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. The skin barrier consists of mucus, primarily comprising highly glycosylated mucins, and the epithelium. Host mucin glycosylation governs interactions with pathogens and stress is associated with impaired epithelial barrier function. We characterized Atlantic salmon skin barrier function during chronic stress (high density) and mucin O-glycosylation changes in response to acute and chronic stress. Fish held at low (LD: 14–30 kg/m3) and high densities (HD: 50-80 kg/m3) were subjected to acute stress 24 h before sampling at 17 and 21 weeks after start of the experiment. Blood parameters indicated primary and secondary stress responses at both sampling points. At the second sampling, skin barrier function towards molecules was reduced in the HD compared to the LD group (Papp mannitol; p < 0.01). Liquid chromatography–mass spectrometry revealed 81 O-glycan structures from the skin. Fish subjected to both chronic and acute stress had an increased proportion of large O-glycan structures. Overall, four of the O-glycan changes have potential as indicators of stress, especially for the combined chronic and acute stress. Stress thus impairs skin barrier function and induces glycosylation changes, which have potential to both affect interactions with pathogens and serve as stress indicators.

By the author/editor: Benktander, John; Sundh, Henrik, 1 ...; Sundell, Kristin ...; Murugan, Abarna ...; Venkatakrishnan, ...; Padra, János T; show more...; Kolarevic, Jelen ...; Terjesen, Bendik ...; Gorissen, Marnix; Lindén, Sara K., ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Cell Biology

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Immunology

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