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An affinity chromat...
An affinity chromatography and glycoproteomics workflow to profile the chondroitin sulfate proteoglycans that interact with malarial VAR2CSA in the placenta and in cancer
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Toledo, A. G. (author)
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Pihl, J. (author)
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Spliid, C. B. (author)
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- Persson, Andrea (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine
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- Nilsson, Jonas, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine
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Pereira, M. A. (author)
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Gustavsson, T. (author)
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Choudhary, S. (author)
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Oo, H. Z. (author)
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Black, P. C. (author)
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Daugaard, M. (author)
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Esko, J. D. (author)
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- Larson, Göran, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine
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Salanti, A. (author)
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Clausen, T. M. (author)
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(creator_code:org_t)
- 2020-04-27
- 2020
- English.
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In: Glycobiology. - : Oxford University Press (OUP). - 0959-6658 .- 1460-2423. ; 30:12, s. 989-1002
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https://academic.oup...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Chondroitin sulfate (CS) is the placental receptor for the VAR2CSA malaria protein, expressed at the surface of infected erythrocytes during Plasmodium falciparum infection. Infected cells adhere to syncytiotrophoblasts or get trapped within the intervillous space by binding to a determinant in a 4-O-sulfated CS chains. However, the exact structure of these glycan sequences remains unclear. VAR2CSA-reactive CS is also expressed by tumor cells, making it an attractive target for cancer diagnosis and therapeutics. The identities of the proteoglycans carrying these modifications in placental and cancer tissues remain poorly characterized. This information is clinically relevant since presentation of the glycan chains may be mediated by novel core proteins or by a limited subset of established proteoglycans. To address this question, VAR2CSA-binding proteoglycans were affinity-purified from the human placenta, tumor tissues and cancer cells and analyzed through a specialized glycoproteomics workflow. We show that VAR2CSA-reactive CS chains associate with a heterogenous group of proteoglycans, including novel core proteins. Additionally, this work demonstrates how affinity purification in combination with glycoproteomics analysis can facilitate the characterization of CSPGs with distinct CS epitopes. A similar workflow can be applied to investigate the interaction of CSPGs with other CS binding lectins as well.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- cancer
- chondroitin sulfate
- glycopeptides
- mass spectrometry
- proteoglycans
- falciparum-infected erythrocytes
- brain natriuretic peptide
- protein
- linkage region
- plasmodium-falciparum
- heparan-sulfate
- glycopeptides
- adherence
- expression
- 4-sulfate
- adhesion
- Biochemistry & Molecular Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Toledo, A. G.
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Pihl, J.
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Spliid, C. B.
-
Persson, Andrea
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Nilsson, Jonas, ...
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Pereira, M. A.
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show more...
-
Gustavsson, T.
-
Choudhary, S.
-
Oo, H. Z.
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Black, P. C.
-
Daugaard, M.
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Esko, J. D.
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Larson, Göran, 1 ...
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Salanti, A.
-
Clausen, T. M.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
- Articles in the publication
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Glycobiology
- By the university
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University of Gothenburg