Hunting monolignol transporters: membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce

Both the mechanisms of monolignol transport and the transported form of monolignols in developing xylem of trees are unknown. We tested the hypothesis of an active, plasma membrane-localized transport of monolignol monomers, dimers, and/or glucosidic forms with membrane vesicles prepared from developing xylem and lignin-forming tissuecultured cells of Norway spruce (Picea abies L. Karst.), as well as from control materials, comprising non-lignifying Norway spruce phloem and tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported both coniferin and p-coumaryl alcohol glucoside, but inhibitor assays suggested that this transport was through the tonoplast. Membrane vesicles prepared from lignin-forming spruce cells showed coniferin transport, but the K-m value for coniferin was much higher than those of xylem and BY-2 cells. Liquid chromatography-mass spectrometry analysis of membrane proteins isolated from spruce developing xylem, phloem, and lignin-forming cultured cells revealed multiple transporters. These were compared with a transporter gene set obtained by a correlation analysis with a selected set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays showed no support for ABC-transporter-mediated monolignol transport but point to a role for secondary active transporters (such as MFS or MATE transporters). In contrast, proteomic and co-expression analyses suggested a role for ABC transporters and MFS transporters.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
LANTBRUKSVETENSKAPER Lantbruksvetenskap, skogsbruk och fiske Trävetenskap hsv//swe
AGRICULTURAL SCIENCES Agriculture, Forestry and Fisheries Wood Science hsv//eng
NATURVETENSKAP Biologi Botanik hsv//swe
NATURAL SCIENCES Biological Sciences Botany hsv//eng
Lignin biosynthesis
monolignol transport
plasma membrane
proteomics
transporter proteins
p-coumaroyl transferase
differentiating xylem
coniferyl-alcohol
lignin precursors
abc transporters
expression analysis
plasma-membranes
cambial sap
lignification
protein
Plant Sciences
Lignin biosynthesis

Takahashi Schmidt, JunkoSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,University of Helsinki(Swepub:slu)47545 (author)
Obudulu, Ogonna,1976Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,University of Gothenburg(Swepub:slu)48345 (author)
Bygdell, JoakimUmeå universitet,Kemiska institutionen(Swepub:umu)jombyl01 (author)
Karhunen, P. (author)
Blokhina, O. (author)
Laitinen, T. (author)
Teeri, T. H. (author)
Wingsle, GunnarSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology(Swepub:slu)48677 (author)
Fagerstedt, K. V. (author)
Karkonen, A. (author)
Sveriges lantbruksuniversitetInstitutionen för skoglig genetik och växtfysiologi (creator_code:org_t)
Sveriges lantbruksuniversitet

In:Journal of Experimental Botany: Oxford University Press (OUP)71:20, s. 6379-63950022-09571460-2431

By the author/editor: Vaisanen, E.; Takahashi Schmid ...; Obudulu, Ogonna, ...; Bygdell, Joakim; Karhunen, P.; Blokhina, O.; show more...; Laitinen, T.; Teeri, T. H.; Wingsle, Gunnar; Fagerstedt, K. V ...; Karkonen, A.; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

AGRICULTURAL SCIENCES: AGRICULTURAL SCI ...; and Agriculture Fore ...; and Wood Science

By the university: University of Gothenburg; Swedish University of Agricultural Sciences; Umeå University

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.