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Hunting monolignol ...
Hunting monolignol transporters: membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce
- Article/chapterEnglish2020
Publisher, publication year, extent ...
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2020-08-10
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Oxford University Press (OUP),2020
Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/301862
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https://gup.ub.gu.se/publication/301862URI
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https://doi.org/10.1093/jxb/eraa368DOI
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https://res.slu.se/id/publ/110297URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-179423URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Both the mechanisms of monolignol transport and the transported form of monolignols in developing xylem of trees are unknown. We tested the hypothesis of an active, plasma membrane-localized transport of monolignol monomers, dimers, and/or glucosidic forms with membrane vesicles prepared from developing xylem and lignin-forming tissuecultured cells of Norway spruce (Picea abies L. Karst.), as well as from control materials, comprising non-lignifying Norway spruce phloem and tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported both coniferin and p-coumaryl alcohol glucoside, but inhibitor assays suggested that this transport was through the tonoplast. Membrane vesicles prepared from lignin-forming spruce cells showed coniferin transport, but the K-m value for coniferin was much higher than those of xylem and BY-2 cells. Liquid chromatography-mass spectrometry analysis of membrane proteins isolated from spruce developing xylem, phloem, and lignin-forming cultured cells revealed multiple transporters. These were compared with a transporter gene set obtained by a correlation analysis with a selected set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays showed no support for ABC-transporter-mediated monolignol transport but point to a role for secondary active transporters (such as MFS or MATE transporters). In contrast, proteomic and co-expression analyses suggested a role for ABC transporters and MFS transporters.
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Takahashi Schmidt, JunkoSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,University of Helsinki(Swepub:slu)47545
(author)
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Obudulu, Ogonna,1976Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,University of Gothenburg(Swepub:slu)48345
(author)
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Bygdell, JoakimUmeå universitet,Kemiska institutionen(Swepub:umu)jombyl01
(author)
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Karhunen, P.
(author)
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Blokhina, O.
(author)
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Laitinen, T.
(author)
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Teeri, T. H.
(author)
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Wingsle, GunnarSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology(Swepub:slu)48677
(author)
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Fagerstedt, K. V.
(author)
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Karkonen, A.
(author)
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Sveriges lantbruksuniversitetInstitutionen för skoglig genetik och växtfysiologi
(creator_code:org_t)
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Sveriges lantbruksuniversitet
Related titles
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In:Journal of Experimental Botany: Oxford University Press (OUP)71:20, s. 6379-63950022-09571460-2431
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Vaisanen, E.
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Takahashi Schmid ...
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Obudulu, Ogonna, ...
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Bygdell, Joakim
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Karhunen, P.
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Blokhina, O.
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Laitinen, T.
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Teeri, T. H.
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Wingsle, Gunnar
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Fagerstedt, K. V ...
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Karkonen, A.
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agriculture Fore ...
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and Wood Science
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NATURAL SCIENCES
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and Biological Scien ...
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and Botany
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University of Gothenburg
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Swedish University of Agricultural Sciences
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Umeå University