Search: onr:"swepub:oai:gup.ub.gu.se/305952" >
Robust generation o...
Robust generation of person-specific, synchronously active neuronal networks using purely isogenic human iPSC-3D neural aggregate cultures
-
- Izsak, Julia (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
-
- Seth, Henrik, 1979 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
-
- Andersson, Mats, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
-
show more...
-
- Vizlin-Hodzic, Dzeneta (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Sahlgrenska Cancer Center,Institute of Neuroscience and Physiology
-
Theiss, Stephan (author)
-
- Hanse, Eric, 1962 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
-
- Ågren, Hans, 1945 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
-
- Funa, Keiko, 1949 (author)
- Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center
-
- Illes, Sebastian (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
-
show less...
-
(creator_code:org_t)
- 2019-04-24
- 2019
- English.
-
In: Frontiers in Neuroscience. - : Frontiers Media SA. - 1662-4548 .- 1662-453X. ; 13
- Related links:
-
https://www.frontier...
-
show more...
-
https://gup.ub.gu.se...
-
https://doi.org/10.3...
-
show less...
Abstract
Subject headings
Close
- Reproducibly generating human induced pluripotent stem cell-based functional neuronal circuits, solely obtained from single individuals, poses particular challenges to achieve personalized and patient specific functional neuronal in vitro models. A hallmark of functional neuronal assemblies, synchronous neuronal activity, can be non-invasively studied by microelectrode array (MEA) technology, reliably capturing physiological and pathophysiological aspects of human brain function. In our here presented manuscript, we demonstrate a procedure to generate 3D neural aggregates comprising astrocytes, oligodendroglial cells, and neurons obtained from the same human tissue sample. Moreover, we demonstrate the robust ability of those neurons to create a highly synchronously active neuronal network within 3 weeks in vitro, without additionally applied astrocytes. The fusion of MEA-technology with functional neuronal circuits solely obtained from one individual's cells represent isogenic person-specific human neuronal sensor chips that pave the way for specific personalized in vitro neuronal networks as well as neurological and neuropsychiatric disease modeling.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Keyword
- 3D-neural model system
- Human induced pluripotent stem cells
- Microelectrode array
- Neuronal networks
- Synchronous activity
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Izsak, Julia
-
Seth, Henrik, 19 ...
-
Andersson, Mats, ...
-
Vizlin-Hodzic, D ...
-
Theiss, Stephan
-
Hanse, Eric, 196 ...
-
show more...
-
Ågren, Hans, 194 ...
-
Funa, Keiko, 194 ...
-
Illes, Sebastian
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Medical Biotechn ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
- Articles in the publication
-
Frontiers in Neu ...
- By the university
-
University of Gothenburg