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TGF-β1 Suppresses P...
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Izsak, JuliaGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
(author)
TGF-β1 Suppresses Proliferation and Induces Differentiation in Human iPSC Neural in vitro Models
- Article/chapterEnglish2020
Publisher, publication year, extent ...
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2020-10-28
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Frontiers Media SA,2020
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LIBRIS-ID:oai:gup.ub.gu.se/305954
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https://gup.ub.gu.se/publication/305954URI
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https://doi.org/10.3389/fcell.2020.571332DOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Persistent neural stem cell (NSC) proliferation is, among others, a hallmark of immaturity in human induced pluripotent stem cell (hiPSC)-based neural models. TGF-β1 is known to regulate NSCs in vivo during embryonic development in rodents. Here we examined the role of TGF-β1 as a potential candidate to promote in vitro differentiation of hiPSCs-derived NSCs and maturation of neuronal progenies. We present that TGF-β1 is specifically present in early phases of human fetal brain development. We applied confocal imaging and electrophysiological assessment in hiPSC-NSC and 3D neural in vitro models and demonstrate that TGF-β1 is a signaling protein, which specifically suppresses proliferation, enhances neuronal and glial differentiation, without effecting neuronal maturation. Moreover, we demonstrate that TGF-β1 is equally efficient in enhancing neuronal differentiation of human NSCs as an artificial synthetic small molecule. The presented approach provides a proof-of-concept to replace artificial small molecules with more physiological signaling factors, which paves the way to improve the physiological relevance of human neural developmental in vitro models.
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Vizlin-Hodzic, DzenetaGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
(author)
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Iljin, MargaritaGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
(author)
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Strandberg, Joakim,1978Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology(Swepub:gu)xstrjo
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Jadasz, Janusz
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Olsson, ThomasGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
(author)
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Theiss, Stephan
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Hanse, EricGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
(author)
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Ågren, HansGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
(author)
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Funa, Keiko,1949Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center(Swepub:gu)xfunke
(author)
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Illes, SebastianGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
(author)
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Göteborgs universitetInstitutionen för neurovetenskap och fysiologi
(creator_code:org_t)
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In:Frontiers in Cell and Developmental Biology: Frontiers Media SA82296-634X
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Izsak, Julia
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Vizlin-Hodzic, D ...
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Iljin, Margarita
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Strandberg, Joak ...
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Jadasz, Janusz
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Olsson, Thomas
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Theiss, Stephan
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Hanse, Eric
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Ågren, Hans
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Funa, Keiko, 194 ...
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Illes, Sebastian
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University of Gothenburg