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High-resolution cry...
High-resolution crystal structures of transient intermediates in the phytochrome photocycle
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Carrillo, M. (author)
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Pandey, S. (author)
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Sanchez, J. (author)
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Noda, M. (author)
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Poudyal, I. (author)
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Aldama, L. (author)
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Malla, T. N. (author)
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- Claesson, Elin, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Wahlgren, Weixiao Yuan, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Feliz, D. (author)
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Srajer, V. (author)
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- Maj, Michal (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Castillon, Leticia (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Iwata, S. (author)
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Nango, E. (author)
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Tanaka, R. (author)
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Tanaka, T. (author)
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Fangjia, L. (author)
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Tono, K. (author)
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Owada, S. (author)
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- Westenhoff, Sebastian, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Stojkovic, E. A. (author)
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Schmidt, M. (author)
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(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- English.
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In: Structure. - : Elsevier BV. - 0969-2126. ; 29:7
- Related links:
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https://www.ncbi.nlm...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Phytochromes are red/far-red light photoreceptors in bacteria to plants, which elicit a variety of important physiological responses. They display a reversible photocycle between the resting Pr state and the light-activated Pfr state. Light signals are transduced as structural change through the entire protein to modulate its activity. It is unknown how the Pr-to-Pfr interconversion occurs, as the structure of intermediates remains notoriously elusive. Here, we present short-lived crystal structures of the photosensory core modules of the bacteriophytochrome from myxobacterium Stigmatella aurantiaca captured by an X-ray free electron laser 5 ns and 33 ms after light illumination of the Pr state. We observe large structural displacements of the covalently bound bilin chromophore, which trigger a bifurcated signaling pathway that extends through the entire protein. The snapshots show with atomic precision how the signal progresses from the chromophore, explaining how plants, bacteria, and fungi sense red light.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- serial femtosecond crystallography
- photoactive yellow protein
- chromophore-binding domain
- fluorescence quantum yield
- signaling
- mechanism
- ligand migration
- bacteriophytochrome
- myoglobin
- nanosecond
- reveals
- Biochemistry & Molecular Biology
- Biophysics
- Cell Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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Structure
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To the university's database
- By the author/editor
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Carrillo, M.
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Pandey, S.
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Sanchez, J.
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Noda, M.
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Poudyal, I.
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Aldama, L.
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show more...
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Malla, T. N.
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Claesson, Elin, ...
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Wahlgren, Weixia ...
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Feliz, D.
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Srajer, V.
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Maj, Michal
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Castillon, Letic ...
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Iwata, S.
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Nango, E.
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Tanaka, R.
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Tanaka, T.
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Fangjia, L.
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Tono, K.
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Owada, S.
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Westenhoff, Seba ...
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Stojkovic, E. A.
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Schmidt, M.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Structure
- By the university
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University of Gothenburg