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Mechanism of NanR g...
Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism
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Horne, C. R. (author)
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Venugopal, H. (author)
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Panjikar, S. (author)
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Wood, D. M. (author)
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Henrickson, A. (author)
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Brookes, E. (author)
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North, R. A. (author)
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Murphy, J. M. (author)
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- Friemann, Rosmarie, 1975 (author)
- Gothenburg University,Göteborgs universitet,CARe - Centrum för antibiotikaresistensforskning,Centre for antibiotic resistance research, CARe
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Griffin, M. D. W. (author)
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Ramm, G. (author)
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Demeler, B. (author)
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Dobson, R. C. J. (author)
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(creator_code:org_t)
- 2021-03-31
- 2021
- English.
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In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 12:1
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https://www.nature.c...
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Abstract
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- Bacteria respond to environmental changes by inducing transcription of some genes and repressing others. Sialic acids, which coat human cell surfaces, are a nutrient source for pathogenic and commensal bacteria. The Escherichia coli GntR-type transcriptional repressor, NanR, regulates sialic acid metabolism, but the mechanism is unclear. Here, we demonstrate that three NanR dimers bind a (GGTATA)(3)-repeat operator cooperatively and with high affinity. Single-particle cryo-electron microscopy structures reveal the DNA-binding domain is reorganized to engage DNA, while three dimers assemble in close proximity across the (GGTATA)(3)-repeat operator. Such an interaction allows cooperative protein-protein interactions between NanR dimers via their N-terminal extensions. The effector, N-acetylneuraminate, binds NanR and attenuates the NanR-DNA interaction. The crystal structure of NanR in complex with N-acetylneuraminate reveals a domain rearrangement upon N-acetylneuraminate binding to lock NanR in a conformation that weakens DNA binding. Our data provide a molecular basis for the regulation of bacterial sialic acid metabolism. The GntR superfamily is one of the largest families of transcription factors in prokaryotes. Here the authors combine biophysical analysis and structural biology to dissect the mechanism by which NanR - a GntR-family regulator - binds to its promoter to repress the transcription of genes necessary for sialic acid metabolism.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Science & Technology - Other Topics
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Horne, C. R.
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Venugopal, H.
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Panjikar, S.
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Wood, D. M.
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Henrickson, A.
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Brookes, E.
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show more...
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North, R. A.
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Murphy, J. M.
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Friemann, Rosmar ...
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Griffin, M. D. W ...
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Ramm, G.
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Demeler, B.
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Dobson, R. C. J.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Nature Communica ...
- By the university
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University of Gothenburg