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The three-dimension...
The three-dimensional structure of Drosophila melanogaster (6-4) photolyase at room temperature
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- Cellini, Andrea, 1991 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Wahlgren, Weixiao Yuan, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Henry, Léocadie (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Pandey, S. (author)
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- Ghosh, Swagatha, 1987 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Castillon, Leticia (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Claesson, Elin, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Takala, H. (author)
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- Kübel, Joachim, 1988 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Nimmrich, Amke, 1995 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Kuznetsova, V. (author)
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Nango, E. (author)
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Iwata, S. (author)
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Owada, S. (author)
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Stojkovic, E. A. (author)
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Schmidt, M. (author)
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Ihalainen, J. A. (author)
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- Westenhoff, Sebastian, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
- 2021-07-29
- 2021
- English.
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In: Acta Crystallographica Section D-Structural Biology. - : International Union of Crystallography (IUCr). - 2059-7983. ; 77, s. 1001-1009
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- (6-4) photolyases are flavoproteins that belong to the photolyase/cryptochrome family. Their function is to repair DNA lesions using visible light. Here, crystal structures of Drosophila melanogaster (6-4) photolyase [Dm(6-4)photolyase] at room and cryogenic temperatures are reported. The room-temperature structure was solved to 2.27 angstrom resolution and was obtained by serial femtosecond crystallography (SFX) using an X-ray free-electron laser. The crystallization and preparation conditions are also reported. The cryogenic structure was solved to 1.79 angstrom resolution using conventional X-ray crystallography. The structures agree with each other, indicating that the structural information obtained from crystallography at cryogenic temperature also applies at room temperature. Furthermore, UV-Vis absorption spectroscopy confirms that Dm(6-4)photolyase is photoactive in the crystals, giving a green light to time-resolved SFX studies on the protein, which can reveal the structural mechanism of the photoactivated protein in DNA repair.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- photolyases
- flavoproteins
- FAD
- serial crystallography
- room-temperature structure
- Drosophila melanogaster
- (6-4) photolyase
- serial femtosecond crystallography
- coli DNA photolyase
- x-ray-diffraction
- crystal-structure
- radical transfer
- repair activity
- mechanism
- cryptochrome
- photoactivation
- reconstitution
- Biochemistry & Molecular Biology
- Biophysics
- Crystallography
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Cellini, Andrea, ...
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Wahlgren, Weixia ...
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Henry, Léocadie
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Pandey, S.
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Ghosh, Swagatha, ...
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Castillon, Letic ...
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show more...
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Claesson, Elin, ...
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Takala, H.
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Kübel, Joachim, ...
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Nimmrich, Amke, ...
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Kuznetsova, V.
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Nango, E.
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Iwata, S.
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Owada, S.
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Stojkovic, E. A.
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Schmidt, M.
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Ihalainen, J. A.
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Westenhoff, Seba ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Acta Crystallogr ...
- By the university
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University of Gothenburg