Plasma ACE2 species are differentially altered in COVID-19 patients

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Plasma ACE2 species are differentially altered in COVID-19 patients

Garcia-Ayllon, M. S. (author)

Moreno-Perez, O. (author)

Garcia-Arriaza, J. (author)

Ramos-Rincon, J. M. (author)

Cortes-Gomez, M. A. (author)

Brinkmalm, Gunnar (author): Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry

Andres, M. (author)

Leon-Ramirez, J. M. (author)

Boix, V. (author)

Gil, J. (author)

Zetterberg, Henrik, 1973 (author): Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry

Esteban, M. (author)

Merino, E. (author)

Saez-Valero, J. (author)

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(creator_code:org_t)

2021
2021
English.
In: FASEB Journal. - 0892-6638. ; 35:8

Related links:: https://gup.ub.gu.se...; show more...; https://doi.org/10.1...; show less...

Journal article (peer-reviewed)

Abstract Subject headings

Studies are needed to identify useful biomarkers to assess the severity and prognosis of COVID-19 disease, caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) virus. Here, we examine the levels of various plasma species of the SARS-CoV-2 host receptor, the angiotensin-converting enzyme 2 (ACE2), in patients at different phases of the infection. Human plasma ACE2 species were characterized by immunoprecipitation and western blotting employing antibodies against the ectodomain and the C-terminal domain, using a recombinant human ACE2 protein as control. In addition, changes in the cleaved and full-length ACE2 species were also examined in serum samples derived from humanized K18-hACE2 mice challenged with a lethal dose of SARS-CoV-2. ACE2 immunoreactivity was present in human plasma as several molecular mass species that probably comprise truncated (70 and 75 kDa) and full-length forms (95, 100, 130, and 170 kDa). COVID-19 patients in the acute phase of infection (n = 46) had significantly decreased levels of ACE2 full-length species, while a truncated 70-kDa form was marginally higher compared with non-disease controls (n = 26). Levels of ACE2 full-length species were in the normal range in patients after a recovery period with an interval of 58-70 days (n = 29), while the 70-kDa species decreased. Levels of the truncated ACE2 species served to discriminate between individuals infected by SARS-CoV-2 and those infected with influenza A virus (n = 17). In conclusion, specific plasma ACE2 species are altered in patients with COVID-19 and these changes normalize during the recovery phase. Alterations in ACE2 species following SARS-CoV-2 infection warrant further investigation regarding their potential usefulness as biomarkers for the disease process and to asses efficacy during vaccination.

Subject headings

NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

ACE2
biomarker
COVID-19
plasma
SARS-CoV-2
angiotensin-converting enzyme
sars-coronavirus
functional receptor
messenger-rna
spike protein
expression
cov
sars-cov-2
tmprss2
cloning
Biochemistry & Molecular Biology
Life Sciences & Biomedicine - Other
Topics
Cell Biology

Publication and Content Type

ref (subject category)
art (subject category)

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By the author/editor: Garcia-Ayllon, M ...; Moreno-Perez, O.; Garcia-Arriaza, ...; Ramos-Rincon, J. ...; Cortes-Gomez, M. ...; Brinkmalm, Gunna ...; show more...; Andres, M.; Leon-Ramirez, J. ...; Boix, V.; Gil, J.; Zetterberg, Henr ...; Esteban, M.; Merino, E.; Saez-Valero, J.; show less...

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

Articles in the publication: FASEB Journal

By the university: University of Gothenburg

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