Abeta CSF LC-MS

• In this chapter, a method using an antibody independent approach based on solid phase extraction (SPE) and liquid chromatography (LC)-tandem mass spectrometry (MS/MS) is described for the analysis of Aβ isoforms in cerebrospinal fluid (CSF). Stable isotope-labeled Aβ peptides are used as internal standards, enabling absolute quantification. A high-resolution quadrupole-Orbitrap hybrid instrument was used for measurements. The method allows quantification of CSF Aβ1-42 between 150 and 4000pg/mL. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Neurosciences (hsv//eng)

Keyword

Alzheimer’s disease

Amyloid beta peptides

Cerebrospinal fluid

Liquid chromatography

Mass spectrometry

Solid phase extraction

amyloid beta protein

amyloid beta protein[1-42]

carbon 13

nitrogen 15

calibration

controlled study

human

liquid chromatography-mass spectrometry

priority journal

protein analysis

protein cerebrospinal fluid level

radiolabeling

Publication and Content Type

vet (subject category)

kap (subject category)