Search: onr:"swepub:oai:gup.ub.gu.se/309336" >
Mapping the Phospho...
Mapping the Phospho-dependent ALK Interactome to Identify Novel Components in ALK Signaling
-
Aboualizadeh, F. (author)
-
Yao, Z. (author)
-
- Guan, Jikui (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
-
show more...
-
Drecun, L. (author)
-
Pathmanathan, S. (author)
-
Snider, J. (author)
-
- Umapathy, Ganesh (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
-
Kotlyar, M. (author)
-
Jurisica, I. (author)
-
- Palmer, Ruth H., 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
-
Stagljar, I. (author)
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- English.
-
In: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836. ; 433:23
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Protein-protein interactions (PPIs) play essential roles in Anaplastic Lymphoma Kinase (ALK) signaling. Systematic characterization of ALK interactors helps elucidate novel ALK signaling mechanisms and may aid in the identification of novel therapeutics targeting related diseases. In this study, we used the Mammalian Membrane Two-Hybrid (MaMTH) system to map the phospho-dependent ALK interactome. By screening a library of 86 SH2 domain-containing full length proteins, 30 novel ALK interactors were identified. Many of their interactions are correlated to ALK phosphorylation activity: oncogenic ALK mutations potentiate the interactions and ALK inhibitors attenuate the interactions. Among the novel interactors, NCK2 was further verified in neuroblastoma cells using co-immunoprecipitation. Modulation of ALK activity by addition of inhibitors lead to concomitant changes in the tyrosine phosphorylation status of NCK2 in neuroblastoma cells, strongly supporting the functionality of the ALK/NCK2 interaction. Our study provides a resource list of potential novel ALK signaling components for further study. (C) 2021 The Authors. Published by Elsevier Ltd.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- receptor tyrosine kinase
- anaplastic lymphoma kinase
- lung-cancer
- npm-alk
- proteins
- adapter
- identification
- complement
- expression
- pathways
- Biochemistry & Molecular Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Aboualizadeh, F.
-
Yao, Z.
-
Guan, Jikui
-
Drecun, L.
-
Pathmanathan, S.
-
Snider, J.
-
show more...
-
Umapathy, Ganesh
-
Kotlyar, M.
-
Jurisica, I.
-
Palmer, Ruth H., ...
-
Stagljar, I.
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biochemistry and ...
- Articles in the publication
-
Journal of Molec ...
- By the university
-
University of Gothenburg