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The androgen recept...
The androgen receptor depends on ligand-binding domain dimerization for transcriptional activation
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El Kharraz, S. (author)
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Dubois, V. (author)
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van Royen, M. E. (author)
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Houtsmuller, A. B. (author)
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Pavlova, E. (author)
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Atanassova, N. (author)
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Nguyen, T. (author)
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Voet, A. (author)
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Eerlings, R. (author)
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Handle, F. (author)
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Prekovic, S. (author)
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Smeets, E. (author)
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Moris, L. (author)
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Devlies, W. (author)
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- Ohlsson, Claes, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Centre for Bone and Arthritis Research,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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- Poutanen, Matti (author)
- Gothenburg University,Göteborgs universitet,Centre for Bone and Arthritis Research,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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Verstrepen, K. J. (author)
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Carmeliet, G. (author)
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Launonen, K. M. (author)
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Helminen, L. (author)
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Palvimo, J. J. (author)
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Libert, C. (author)
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Vanderschueren, D. (author)
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Helsen, C. (author)
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Claessens, F. (author)
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(creator_code:org_t)
- 2021-10-18
- 2021
- English.
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In: Embo Reports. - : EMBO. - 1469-221X .- 1469-3178. ; 22:12
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Abstract
Subject headings
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- Whereas dimerization of the DNA-binding domain of the androgen receptor (AR) plays an evident role in recognizing bipartite response elements, the contribution of the dimerization of the ligand-binding domain (LBD) to the correct functioning of the AR remains unclear. Here, we describe a mouse model with disrupted dimerization of the AR LBD (AR(Lmon/Y)). The disruptive effect of the mutation is demonstrated by the feminized phenotype, absence of male accessory sex glands, and strongly affected spermatogenesis, despite high circulating levels of testosterone. Testosterone replacement studies in orchidectomized mice demonstrate that androgen-regulated transcriptomes in AR(Lmon/Y) mice are completely lost. The mutated AR still translocates to the nucleus and binds chromatin, but does not bind to specific AR binding sites. In vitro studies reveal that the mutation in the LBD dimer interface also affects other AR functions such as DNA binding, ligand binding, and co-regulator binding. In conclusion, LBD dimerization is crucial for the development of AR-dependent tissues through its role in transcriptional regulation in vivo. Our findings identify AR LBD dimerization as a possible target for AR inhibition.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- androgen receptor
- chromatin binding
- dimerization
- ligand-binding
- domain
- transcriptional activation
- protein-protein interactions
- insensitivity syndrome
- response element
- structural basis
- knockout
- mass
- estrogen
- spermatogenesis
- reveals
- roles
- Biochemistry & Molecular Biology
- Cell Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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El Kharraz, S.
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Dubois, V.
-
van Royen, M. E.
-
Houtsmuller, A. ...
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Pavlova, E.
-
Atanassova, N.
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show more...
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Nguyen, T.
-
Voet, A.
-
Eerlings, R.
-
Handle, F.
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Prekovic, S.
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Smeets, E.
-
Moris, L.
-
Devlies, W.
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Ohlsson, Claes, ...
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Poutanen, Matti
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Verstrepen, K. J ...
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Carmeliet, G.
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Launonen, K. M.
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Helminen, L.
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Palvimo, J. J.
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Libert, C.
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Vanderschueren, ...
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Helsen, C.
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Claessens, F.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Embo Reports
- By the university
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University of Gothenburg