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Novel colistin-EDTA...
Novel colistin-EDTA combination for successful eradication of colistin-resistant Klebsiella pneumoniae catheter-related biofilm infections
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Shein, A. M. S. (author)
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Wannigama, D. L. (author)
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Higgins, P. G. (author)
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Hurst, C. (author)
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Abe, S. (author)
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Hongsing, P. (author)
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Chantaravisoot, N. (author)
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Saethang, T. (author)
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Luk-in, S. (author)
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Liao, T. T. (author)
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Nilgate, S. (author)
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Rirerm, U. (author)
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Kueakulpattana, N. (author)
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Laowansiri, M. (author)
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Srisakul, S. (author)
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Muhummudaree, N. (author)
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Techawiwattanaboon, T. (author)
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Gan, L. (author)
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Xu, C. C. (author)
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Kupwiwat, R. (author)
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- Phattharapornjaroen, Phatthranit (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery
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Rojanathanes, R. (author)
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Leelahavanichkul, A. (author)
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Chatsuwan, T. (author)
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(creator_code:org_t)
- 2021-11-04
- 2021
- English.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 11:1
- Related links:
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https://www.nature.c...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Development of an effective therapy to overcome colistin resistance in Klebsiella pneumoniae, a common pathogen causing catheter-related biofilm infections in vascular catheters, has become a serious therapeutic challenge that must be addressed urgently. Although colistin and EDTA have successful roles for eradicating biofilms, no in vitro and in vivo studies have investigated their efficacy in catheter-related biofilm infections of colistin-resistant K. pneumoniae. In this study, colistin resistance was significantly reversed in both planktonic and mature biofilms of colistin-resistant K. pneumoniae by a combination of colistin (0.25-1 mu g/ml) with EDTA (12 mg/ml). This novel colistin-EDTA combination was also demonstrated to have potent efficacy in eradicating colistin-resistant K. pneumoniae catheter-related biofilm infections, and eliminating the risk of recurrence in vivo. Furthermore, this study revealed significant therapeutic efficacy of colistin-EDTA combination in reducing bacterial load in internal organs, lowering serum creatinine, and protecting treated mice from mortality. Altered in vivo expression of different virulence genes indicate bacterial adaptive responses to survive in hostile environments under different treatments. According to these data discovered in this study, a novel colistin-EDTA combination provides favorable efficacy and safety for successful eradication of colistin-resistant K. pneumonia catheter-related biofilm infections.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Keyword
- pseudomonas-aeruginosa
- escherichia-coli
- lock solution
- calcium-edta
- gentamicin
- vancomycin
- diagnosis
- spectrum
- therapy
- update
- Science & Technology - Other Topics
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Shein, A. M. S.
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Wannigama, D. L.
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Higgins, P. G.
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Hurst, C.
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Abe, S.
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Hongsing, P.
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show more...
-
Chantaravisoot, ...
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Saethang, T.
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Luk-in, S.
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Liao, T. T.
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Nilgate, S.
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Rirerm, U.
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Kueakulpattana, ...
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Laowansiri, M.
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Srisakul, S.
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Muhummudaree, N.
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Techawiwattanabo ...
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Gan, L.
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Xu, C. C.
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Kupwiwat, R.
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Phattharapornjar ...
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Rojanathanes, R.
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Leelahavanichkul ...
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Chatsuwan, T.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
- Articles in the publication
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Scientific Repor ...
- By the university
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University of Gothenburg