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A luminescence-base...
A luminescence-based protocol for assessing fructose metabolism via quantification of ketohexokinase enzymatic activity in mouse or human hepatocytes
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Park, Se Hyung (author)
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Helsley, Robert N. (author)
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Noetzli, Leila (author)
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Tu, Ho Chou (author)
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Wallenius, Kristina (author)
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O'Mahony, Gavin (author)
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- Boucher, Jeremie (author)
- Gothenburg University,Göteborgs universitet,Wallenberg Centre for Molecular and Translational Medicine
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Liu, Jianming (author)
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Softic, Samir (author)
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(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- English.
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In: STAR Protocols. - : Elsevier BV. - 2666-1667. ; 2:3
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https://doi.org/10.1...
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Abstract
Subject headings
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- Ketohexokinase (KHK) catalyzes the first step of fructose metabolism. Inhibitors of KHK enzymatic activity are being evaluated in clinical trials for the treatment of non-alcoholic fatty liver disease (NAFLD) and diabetes. Here, we present a luminescence-based protocol to quantify KHK activity. The accuracy of this technique has been validated using knockdown and overexpression of KHK in vivo and in vitro. The specificity of the assay has been verified using 3-O-methyl-D-fructose, a non-metabolizable analog of fructose, heat inactivation of hexokinases, and depletion of potassium. For complete details on the use of this protocol, please refer to Damen et al. (2021).
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- Cell Biology
- Cell-based assays
- Metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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