Aborting meiosis allows recombination in sterile diploid yeast hybrids

Hybrids are often considered evolutionary dead ends because they do not generate viable offspring. Here, the authors show that sterile yeast hybrids generate genetic diversity through meiotic-like recombination by aborting meiosis and return to asexual growth. Hybrids between diverged lineages contain novel genetic combinations but an impaired meiosis often makes them evolutionary dead ends. Here, we explore to what extent an aborted meiosis followed by a return-to-growth (RTG) promotes recombination across a panel of 20 Saccharomyces cerevisiae and S. paradoxus diploid hybrids with different genomic structures and levels of sterility. Genome analyses of 275 clones reveal that RTG promotes recombination and generates extensive regions of loss-of-heterozygosity in sterile hybrids with either a defective meiosis or a heavily rearranged karyotype, whereas RTG recombination is reduced by high sequence divergence between parental subgenomes. The RTG recombination preferentially arises in regions with low local heterozygosity and near meiotic recombination hotspots. The loss-of-heterozygosity has a profound impact on sexual and asexual fitness, and enables genetic mapping of phenotypic differences in sterile lineages where linkage analysis would fail. We propose that RTG gives sterile yeast hybrids access to a natural route for genome recombination and adaptation.

Tattini, L.Université Côte d'Azur,University of Côte d'Azur (author)
Llored, A.Université Côte d'Azur,University of Côte d'Azur (author)
Irizar, A.Université Côte d'Azur,University of Côte d'Azur (author)
Skofljanc, N.Université Côte d'Azur,University of Côte d'Azur (author)
D'Angiolo, M.Université Côte d'Azur,University of Côte d'Azur (author)
De Chiara, M.Université Côte d'Azur,University of Côte d'Azur (author)
Barre, B. P.Université Côte d'Azur,University of Côte d'Azur (author)
Yue, J. X.Sun Yat-Sen University (author)
Lutazi, A.Université Côte d'Azur,University of Côte d'Azur (author)
Loeillet, S. (author)
Laureau, R.Columbia University in the City of New York (author)
Marsit, S. (author)
Stenberg, S.Chalmers tekniska högskola,Chalmers University of Technology (author)
Albaud, B. (author)
Persson, Karl,1988Göteborgs universitet,University of Gothenburg(Swepub:cth)karperss (author)
Legras, J. L.Université de Montpellier,University of Montpellier (author)
Dequin, S.Université de Montpellier,University of Montpellier (author)
Warringer, Jonas,1973Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:gu)xwarrj (author)
Nicolas, A.Université Côte d'Azur,University of Côte d'Azur (author)
Liti, G.Université Côte d'Azur,University of Côte d'Azur (author)
Université Côte d'AzurSun Yat-Sen University (creator_code:org_t)

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Evolutionary Bio ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Cell Biology

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Microbiology

By the university: University of Gothenburg; Chalmers University of Technology

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