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Simultaneous Counti...
Simultaneous Counting of Molecules in the Halo and Dense-Core of Nanovesicles by Regulating Dynamics of Vesicle Opening
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- He, Xiulan (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Ewing, Andrew G, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
- 2022-02-18
- 2022
- English.
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In: Angewandte Chemie-International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 61:15
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Abstract
Subject headings
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- We report the discovery that in the presence of chaotropic anions (SCN-) the opening of nanometer biological vesicles at an electrified interface often becomes a two-step process (around 30 % doublet peaks). We have then used this to independently count molecules in each subvesicular compartment, the halo and protein dense-core, and the fraction of catecholamine binding to the dense-core is 68 %. Moreover, we differentiated two distinct populations of large dense-core vesicles (LDCVs) and quantified their content, which might correspond to immature (43 %) and mature (30 %) LDCVs, to reveal differences in their biogenesis. We speculate this is caused by an increase in the electrostatic attraction between protonated catecholamine and the negatively charged dense-core following adsorption of SCN-.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Amperometry
- Chaotropic Effect
- Hofmeister Series
- Large Dense-Core
- Vesicles
- Vesicle Opening
- secretory granule
- catecholamine content
- vesicular exocytosis
- ph
- cells
- size
- transmitters
- populations
- mechanisms
- kinetics
- Chemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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