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- He, XiulanGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology (author)
Simultaneous Counting of Molecules in the Halo and Dense-Core of Nanovesicles by Regulating Dynamics of Vesicle Opening
- Article/chapterEnglish2022
Publisher, publication year, extent ...
- 2022-02-18
- Wiley,2022
Numbers
- LIBRIS-ID:oai:gup.ub.gu.se/314086
- https://gup.ub.gu.se/publication/314086URI
- https://doi.org/10.1002/anie.202116217DOI
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- Language:English
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- We report the discovery that in the presence of chaotropic anions (SCN-) the opening of nanometer biological vesicles at an electrified interface often becomes a two-step process (around 30 % doublet peaks). We have then used this to independently count molecules in each subvesicular compartment, the halo and protein dense-core, and the fraction of catecholamine binding to the dense-core is 68 %. Moreover, we differentiated two distinct populations of large dense-core vesicles (LDCVs) and quantified their content, which might correspond to immature (43 %) and mature (30 %) LDCVs, to reveal differences in their biogenesis. We speculate this is caused by an increase in the electrostatic attraction between protonated catecholamine and the negatively charged dense-core following adsorption of SCN-.
Subject headings and genre
- NATURVETENSKAP Kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences hsv//eng
- Amperometry
- Chaotropic Effect
- Hofmeister Series
- Large Dense-Core
- Vesicles
- Vesicle Opening
- secretory granule
- catecholamine content
- vesicular exocytosis
- ph
- cells
- size
- transmitters
- populations
- mechanisms
- kinetics
- Chemistry
Added entries (persons, corporate bodies, meetings, titles ...)
- Ewing, Andrew G,1957Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xewian (author)
- Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)
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- In:Angewandte Chemie-International Edition: Wiley61:151433-78511521-3773
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