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Assessing the effec...
Assessing the effectiveness of percussive and electrical stunning in rainbow trout: Does an epileptic-like seizure imply brain failure?
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- Hjelmstedt, P. (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens miljö och hälsa (HMH),Department of Animal Environment and Health
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- Sundell, E. (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens miljö och hälsa (HMH),Department of Animal Environment and Health
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Brijs, J. (author)
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Berg, C. (author)
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- Sandblom, Erik, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences
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Lines, J. (author)
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- Axelsson, Michael, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences
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- Gräns, Albin (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens miljö och hälsa (HMH),Department of Animal Environment and Health
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- Berg, Lotta (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens miljö och hälsa (HMH),Department of Animal Environment and Health
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(creator_code:org_t)
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- Elsevier BV, 2022
- 2022
- English.
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In: Aquaculture. - : Elsevier BV. - 0044-8486 .- 1873-5622. ; 552
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Abstract
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- Both percussive and electrical stunning have been highlighted as methods that can be used to quickly render fish unconscious before being killed. However, accurately assessing unconsciousness in animals following stunning remains challenging, and thus methods for reliable interpretation and validation of different stunning methods are urgently needed. Here, we used a non-invasive technique to continuously record electroencephalograms (EEG) of rainbow trout (Oncorhynchus mykiss) enabling us to compare the effects of both percussive stunning, using a captive bolt gun, and various combinations of electrical stun parameters delivered in water. The EEG signals were assessed for the absence or presence of an epileptic-like seizure and for visually evoked responses (VERs). No epileptic-like seizures or VERs were observed after captive bolt stunning. We found that it is possible to reliably induce an epileptic-like seizure and an immediate, but short lasting, loss of VERs following a 1 s exposure to an electrical field strength of at least 2.8 VRMS cm−1 and current density of 0.22 ARMS dm−2 in water of conductivity of ~1000 μS cm−1 using a 50 Hz AC current. However, to avoid recovery of VERs shortly after the stun, it was necessary to increase the duration of the stun application (≥30 s), the electrical field strength (10.2 VRMS cm−1) and the current density (0.84 ARMS dm−2 respectively). We found no clear relationship between presence and absence of ventilation and VERs following electrical stunning in rainbow trout, highlighting that loss of ventilation may not be a good indicator of brain failure in rainbow trout. Our results clearly show that the presence of an epileptic-like seizure following an electrical stun does not guarantee a prolonged period where the fish is unresponsive to visual stimulation (i.e. absence of VERs). It was further found that VERs can return before the end of the seizure. As both presence of a seizure and absence of VERs have been used independently as indicators of unconsciousness in fish, we emphasize the necessity to carefully consider and evaluate the reliability of neurophysiological indicators of unconsciousness when validating methods to stun fish. © 2022
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Fisk- och akvakulturforskning (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Fish and Aquacultural Science (hsv//eng)
Keyword
- EEG
- Slaughter
- Tonic-clonic
- Visually evoked response
- Welfare
- assessment method
- biostimulation
- brain
- neurology
- perciform
- ventilation
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Hjelmstedt, P.
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Sundell, E.
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Brijs, J.
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Berg, C.
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Sandblom, Erik, ...
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Lines, J.
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show more...
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Axelsson, Michae ...
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Gräns, Albin
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Berg, Lotta
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agriculture Fore ...
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and Fish and Aquacul ...
- Articles in the publication
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Aquaculture
- By the university
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University of Gothenburg
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Swedish University of Agricultural Sciences