Search: onr:"swepub:oai:gup.ub.gu.se/314636" >
Stress-induced tyro...
Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs
-
Papaioannou, A. (author)
-
Centonze, F. (author)
-
Metais, A. (author)
-
show more...
-
Maurel, M. (author)
-
Negroni, L. (author)
-
Gonzalez-Quiroz, M. (author)
-
- Mahdizadeh, Sayyed Jalil (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
- Svensson, Gabriella (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
- Zare, Ensieh (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Blondel, A. (author)
-
Koong, A. C. (author)
-
Hetz, C. (author)
-
Pedeux, R. (author)
-
Tremblay, M. L. (author)
-
- Eriksson, Leif A, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Chevet, E. (author)
-
show less...
-
(creator_code:org_t)
- 2022-02-22
- 2022
- English.
-
In: Life science alliance. - : Life Science Alliance, LLC. - 2575-1077. ; 5:5
- Related links:
-
https://www.life-sci...
-
show more...
-
https://gup.ub.gu.se...
-
https://doi.org/10.2...
-
show less...
Abstract
Subject headings
Close
- ER stress is mediated by three sensors and the most evolutionary conserved IRE1α signals through its cytosolic kinase and endoribonuclease (RNase) activities. IRE1α RNase activity can either catalyze the initial step of XBP1 mRNA unconventional splicing or degrade a number of RNAs through regulated IRE1-dependent decay. Until now, the biochemical and biological outputs of IRE1α RNase activity have been well documented; however, the precise mechanisms controlling whether IRE1α signaling is adaptive or pro-death (terminal) remain unclear. We investigated those mechanisms and hypothesized that XBP1 mRNA splicing and regulated IRE1-dependent decay activity could be co-regulated by the IRE1α RNase regulatory network. We identified that RtcB, the tRNA ligase responsible for XBP1 mRNA splicing, is tyrosine-phosphorylated by c-Abl and dephosphorylated by PTP1B. Moreover, we show that the phosphorylation of RtcB at Y306 perturbs RtcB interaction with IRE1α, thereby attenuating XBP1 mRNA splicing. Our results demonstrate that the IRE1α RNase regulatory network is dynamically fine-tuned by tyrosine kinases and phosphatases upon various stresses and that the extent of RtcB tyrosine phosphorylation determines cell adaptive or death outputs. © 2022 Papaioannou et al.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Papaioannou, A.
-
Centonze, F.
-
Metais, A.
-
Maurel, M.
-
Negroni, L.
-
Gonzalez-Quiroz, ...
-
show more...
-
Mahdizadeh, Sayy ...
-
Svensson, Gabrie ...
-
Zare, Ensieh
-
Blondel, A.
-
Koong, A. C.
-
Hetz, C.
-
Pedeux, R.
-
Tremblay, M. L.
-
Eriksson, Leif A ...
-
Chevet, E.
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biochemistry and ...
- Articles in the publication
-
Life science all ...
- By the university
-
University of Gothenburg