eIF6 rebinding dynamically couples ribosome maturation and translation.

Jaako, Pekka (author): Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Extern,Institute of Biomedicine,External

Faille, Alexandre (author)

Tan, Shengjiang (author)

Wong, Chi C (author)

Escudero-Urquijo, Norberto (author)

Castro-Hartmann, Pablo (author)

Wright, Penny (author)

Hilcenko, Christine (author)

Adams, David J (author)

Warren, Alan J (author)

(creator_code:org_t)

2022-03-23
2022
English.
In: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 13:1

Related links:: https://gup.ub.gu.se...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

Protein synthesis is a cyclical process consisting of translation initiation, elongation, termination and ribosome recycling. The release factors SBDS and EFL1-both mutated in the leukemia predisposition disorder Shwachman-Diamond syndrome - license entry of nascent 60S ribosomal subunits into active translation by evicting the anti-association factor eIF6 from the 60S intersubunit face. We find that in mammalian cells, eIF6 holds all free cytoplasmic 60S subunits in a translationally inactive state and that SBDS and EFL1 are the minimal components required to recycle these 60S subunits back into additional rounds of translation by evicting eIF6. Increasing the dose of eIF6 in mice in vivo impairs terminal erythropoiesis by sequestering post-termination 60S subunits in the cytoplasm, disrupting subunit joining and attenuating global protein synthesis. These data reveal that ribosome maturation and recycling are dynamically coupled by a mechanism that is disrupted in an inherited leukemia predisposition disorder.

By the author/editor: Jaako, Pekka; Faille, Alexandr ...; Tan, Shengjiang; Wong, Chi C; Escudero-Urquijo ...; Castro-Hartmann, ...; show more...; Wright, Penny; Hilcenko, Christ ...; Adams, David J; Warren, Alan J; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.