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Extracellular endos...
Extracellular endosulfatase Sulf-2 harbors a chondroitin/dermatan sulfate chain that modulates its enzyme activity
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El Masri, R. (author)
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Seffouh, A. (author)
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Roelants, C. (author)
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Seffouh, I. (author)
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Gout, E. (author)
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Perard, J. (author)
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Dalonneau, F. (author)
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Nishitsuji, K. (author)
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- Noborn, Fredrik (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine
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- Nikpour, Mahnaz, 1980 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine
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- Larson, Göran, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine
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Cretinon, Y. (author)
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Friedel-Arboleas, M. (author)
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Uchimura, K. (author)
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Daniel, R. (author)
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Lortat-Jacob, H. (author)
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Filhol, O. (author)
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Vives, R. R. (author)
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(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- English.
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In: Cell Reports. - : Elsevier BV. - 2211-1247. ; 38:11
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Sulfs represent a class of unconventional sulfatases which provide an original post-synthetic regulatory mechanism for heparan sulfate polysaccharides and are involved in multiple physiopathological processes, including cancer. However, Sulfs remain poorly characterized enzymes, with major discrepancies regarding their in vivo functions. Here we show that human SuIf-2 (HSulf-2) harbors a chondroitin/dermatan sulfate glycosaminoglycan (GAG) chain, attached to the enzyme substrate-binding domain. We demonstrate that this GAG chain affects enzyme/substrate recognition and tunes HSulf-2 activity in vitro and in vivo. In addition, we show that mammalian hyaluronidase acts as a promoter of HSulf-2 activity by digesting its GAG chain. In conclusion, our results highlight HSulf-2 as a proteoglycan-related enzyme and its GAG chain as a critical non-catalytic modulator of the enzyme activity. These findings contribute to clarifying the conflicting data on the activities of the Sulfs.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Keyword
- heparan-sulfate
- substrate-specificity
- hyaluronidase
- identification
- metastasis
- mechanism
- linkage
- Cell Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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El Masri, R.
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Seffouh, A.
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Roelants, C.
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Seffouh, I.
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Gout, E.
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Perard, J.
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show more...
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Dalonneau, F.
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Nishitsuji, K.
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Noborn, Fredrik
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Nikpour, Mahnaz, ...
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Larson, Göran, 1 ...
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Cretinon, Y.
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Friedel-Arboleas ...
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Uchimura, K.
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Daniel, R.
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Lortat-Jacob, H.
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Filhol, O.
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Vives, R. R.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomedical Labor ...
- Articles in the publication
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Cell Reports
- By the university
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University of Gothenburg