Search: onr:"swepub:oai:gup.ub.gu.se/316879" >
Dysfunction of vesi...
Dysfunction of vesicular storage in young-onset Parkinson's patient-derived dopaminergic neurons and organoids revealed by single cell electrochemical cytometry
-
Zhu, Wanying (author)
-
Tao, Mengdan (author)
-
Hong, Yuan (author)
-
show more...
-
Wu, Shanshan (author)
-
Chu, Chu (author)
-
Zheng, Zhilong (author)
-
Han, Xiao (author)
-
Zhu, Qian (author)
-
Xu, Min (author)
-
- Ewing, Andrew G, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Guo, Xing (author)
-
Liu, Yan (author)
-
show less...
-
(creator_code:org_t)
- 2022
- 2022
- English.
-
In: Chemical Science. - 2041-6520. ; 13:21, s. 6217-6223
- Related links:
-
https://gup.ub.gu.se...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Electrochemical cytometry based on nano-tip microelectrodes was used to quantify the vesicular storage at the single-cell level in human neurons and midbrain organoids which acted as disease models of young-onset Parkinson's disease (YOPD). Human dopaminergic (DA) neurons and midbrain organoids were derived from an induced pluripotent stem cell line from one YOPD patient. We show a significant deficiency in vesicular catecholamine storage and a slower pore forming process on the surface of the microelectrode in the DA neurons derived from the YOPD patient. The upregulation of α-synuclein in both neurons and organoids derived from the YOPD patient is associated with vesicular storage dysfunction, revealing a correlation between the pathogenesis of YOPD and vesicular chemical storage deficiency, a novel chemical insight into the potential pathology of YOPD. Notably, efficacy evaluation and drug testing were performed with our platform to demonstrate that both amantadine, a clinical drug for Parkinson's disease (PD), and phorbol 12-myristate 13-acetate, an attractive candidate, ameliorate the dysfunction of vesicular storage in DA neurons derived from the YOPD patient. Our platform offers promising avenues for new drug discovery for PD and other neurodegenerative disorders.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Zhu, Wanying
-
Tao, Mengdan
-
Hong, Yuan
-
Wu, Shanshan
-
Chu, Chu
-
Zheng, Zhilong
-
show more...
-
Han, Xiao
-
Zhu, Qian
-
Xu, Min
-
Ewing, Andrew G, ...
-
Guo, Xing
-
Liu, Yan
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biochemistry and ...
- Articles in the publication
-
Chemical Science
- By the university
-
University of Gothenburg