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Conditioned serum i...
Conditioned serum in vitro treatment of chondrocyte pellets and osteoarthritic explants
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- Löfgren, Maria (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health
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- Ekman, Stina (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health
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- Ekholm, Josefine (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
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- Engström, Mona (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
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Fjordbakk, C. T. (author)
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- Svala, Emilia (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health
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- Holm Forsström, Karin (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,University Animal Hospital
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- Lindahl, Anders, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
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- Skiöldebrand, Eva (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,Sahlgrenska University Hospital
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(creator_code:org_t)
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- 2022-06-06
- 2023
- English.
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In: Equine Veterinary Journal. - : Wiley. - 0425-1644 .- 2042-3306. ; 55:2, s. 325-335
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Abstract
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- Background: Autologous conditioned serum (ACS) is used to treat osteoarthritis in horses, although its effects are not fully investigated. Objectives: To investigate the effects of equine serum and conditioned serum on chondrocytes stimulated with interleukin (IL)-1 beta and cartilage explants with mild osteoarthritis. Study design: In vitro experimental study. Methods: The effect of three different serum preparations (unincubated control [PS], serum incubated 24 h [PS24h] and serum incubated 24 h in ACS containers [PCS]) pooled from lame horses were tested in two in vitro models. IL-1 beta and IL-1 receptor antagonist (IL-1Ra) concentrations were measured in all sera. In model 1, chondrocyte pellet cultures were stimulated with IL-1 beta prior to treatment with the serum preparations for 2 and 48 h. Microarray, polymerase chain reaction, and matrix metallopeptidase-13 analyses were performed. In model 2, cartilage explants from horses with structural osteoarthritis were treated with PS or PCS on days 0, 6 and 12, or left untreated, and evaluated at day 24 using the OARSI grading scale for histological evaluation of articular cartilage. Results: The IL-1Ra concentration in PS24h and PCS was significantly higher than in PS. In model 1, inflammation- and cartilage matrix degradation-related genes were upregulated after 48 h in all treatment groups versus untreated controls. Cartilage matrix molecules, aggrecan and collagens, were downregulated in PS24h- and PCS-treated pellets versus untreated controls. Growth factor signalling genes were upregulated-FGF7 in all treatment groups, BMP2 in PS24h-, and INHBA in PCS-treated-compared with untreated controls. In model 2, the OARSI score at day 24 was not significantly different between treatment groups. Main limitations: Results from in vitro models cannot be directly translated to in vivo situations. Conclusions: In vitro treatment with conditioned serum did not alleviate IL-1 beta-induced responses in chondrocyte pellets or lead to morphological improvement in osteoarthritic cartilage explants.
Subject headings
- LANTBRUKSVETENSKAPER -- Veterinärmedicin (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science (hsv//eng)
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Klinisk vetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Clinical Science (hsv//eng)
Keyword
- autologous conditioned serum
- horse
- IL-1 receptor antagonist
- inflammation
- interleukin 1 beta
- osteoarthritis
- growth-factor
- gene-expression
- articular chondrocytes
- equine
- osteoarthritis
- tgf-beta
- cartilage
- receptor
- interleukin-1-beta
- il-1-beta
- cytokines
- Veterinary Sciences
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Löfgren, Maria
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Ekman, Stina
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Ekholm, Josefine
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Engström, Mona
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Fjordbakk, C. T.
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Svala, Emilia
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show more...
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Holm Forsström, ...
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Lindahl, Anders, ...
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Skiöldebrand, Ev ...
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- About the subject
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Veterinary Scien ...
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Veterinary Scien ...
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and Clinical Science
- Articles in the publication
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Equine Veterinar ...
- By the university
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University of Gothenburg
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Swedish University of Agricultural Sciences