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Ring Trial on Quant...
Ring Trial on Quantitative Assessment of Bile Acids Reveals a Method- and Analyte-Specific Accuracy and Reproducibility
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Haange, S. B. (author)
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Till, A. (author)
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- Bergh, Per-Olof (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberglaboratoriet,Institute of Medicine, Department of Molecular and Clinical Medicine,Wallenberg Laboratory
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Fauler, G. (author)
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Gigl, M. (author)
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Lofgren-Sandblom, A. (author)
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Schaap, F. G. (author)
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Clavel, T. (author)
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Trautwein, C. (author)
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Fenske, W. (author)
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Kleigrewe, K. (author)
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- Marschall, Hanns-Ulrich, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberglaboratoriet,Institute of Medicine, Department of Molecular and Clinical Medicine,Wallenberg Laboratory
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Damink, Swmo (author)
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Moustafa, T. (author)
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von Bergen, M. (author)
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Rolle-Kampczyk, U. (author)
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(creator_code:org_t)
- 2022-06-23
- 2022
- English.
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In: Metabolites. - : MDPI AG. - 2218-1989. ; 12:7
- Related links:
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https://doi.org/10.3...
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Abstract
Subject headings
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- Bile acids are a key mediator of the molecular microbiome-host interaction, and various mass spectrometry-based assays have been developed in the recent decade to quantify a wide range of bile acids. We compare existing methodologies to harmonize them. Methodology for absolute quantification of bile acids from six laboratories in Europe were compared for the quantification of the primary bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) and conjugated products glycocholic acid (GCA) and taurocholic acid (TCA). For the bacterially modified secondary bile acids, the quantification of deoxycholic acid (DCA) and lithocholic acid (LCA) was compared. For the murine bile acids, we used the primary muricholic acids (alpha-MCA and, beta-MCA) and the intestinally produced secondary bile acid muricholic (omega-MCA). The standards were spiked into methanol:water (1:1) mix as well as in human and murine serum at either low concentration range (150-3000 nM) or high concentration range (1500-40,000 nM). The precision was better for higher concentrations. Measurements for the hydrophobic unconjugated bile acids LCA and omega-MCA were the most challenging. The quality assessments were generally very similar, and the comprehensive analyses demonstrated that data from chosen locations can be used for comparisons between studies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- bile acids
- ring trial
- LC-MS/MS
- absolute quantification
- human serum
- murine serum
- tandem mass-spectrometry
- quantification
- serum
- Biochemistry & Molecular Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Haange, S. B.
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Till, A.
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Bergh, Per-Olof
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Fauler, G.
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Gigl, M.
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Lofgren-Sandblom ...
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show more...
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Schaap, F. G.
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Clavel, T.
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Trautwein, C.
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Fenske, W.
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Kleigrewe, K.
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Marschall, Hanns ...
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Damink, Swmo
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Moustafa, T.
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von Bergen, M.
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Rolle-Kampczyk, ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Metabolites
- By the university
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University of Gothenburg
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Karolinska Institutet