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Dataset for suppres...
Dataset for suppressors of amyloid-beta toxicity and their functions in recombinant protein production in yeast
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- Chen, Xin, 1980 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Li, Xiaowei, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ji, Boyang, 1983 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Wang, Yanyan, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ishchuk, Olena, 1980 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Vorontsov, Egor, 1988 (author)
- Gothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics,University of Gothenburg
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- Petranovic Nielsen, Dina, 1975 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Siewers, Verena, 1976 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Engqvist, Martin, 1983 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- English.
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In: Data in Brief. - : Elsevier BV. - 2352-3409. ; 42
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Abstract
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- The production of recombinant proteins at high levels often induces stress-related phenotypes by protein misfolding or aggregation. These are similar to those of the yeast Alzheimer's disease (AD) model in which amyloid-beta peptides (A beta 42) were accumulated [1,2] . We have previously identified suppressors of A beta 42 cytotoxicity via the genome-wide synthetic genetic array (SGA) [3] and here we use them as metabolic engineering targets to evaluate their potentiality on recombinant protein production in yeast Saccharomyces cerevisiae. In order to investigate the mechanisms linking the genetic modifications to the improved recombinant protein production, we perform systems biology approaches (transcriptomics and proteomics) on the resulting strain and intermediate strains. The RNAseq data are preprocessed by the nf-core/RNAseq pipeline and analyzed using the Platform for Integrative Analysis of Omics (PIANO) package [4] . The quantitative proteome is analyzed on an Orbitrap Fusion Lumos mass spectrometer interfaced with an Easy-nLC1200 liquid chromatography (LC) system. LC-MS data files are processed by Proteome Discoverer version 2.4 with Mascot 2.5.1 as a database search engine. The original data presented in this work can be found in the research paper titled "Suppressors of Amyloid-beta Toxicity Improve Recombinant Protein Produc-tion in yeast by Reducing Oxidative Stress and Tuning Cellu-lar Metabolism", by Chen et al. [5] . (C) 2022 The Author(s). Published by Elsevier Inc.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Annan medicin och hälsovetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Other Medical and Health Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Keyword
- Amyloid-beta
- Gene engineering
- Transcriptome
- Proteome
- Yeast
- Saccharomyces cerevisiae
- expression
- secretion
- Science & Technology - Other Topics
- Gene engineering
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Chen, Xin, 1980
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Li, Xiaowei, 198 ...
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Ji, Boyang, 1983
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Wang, Yanyan, 19 ...
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Ishchuk, Olena, ...
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Vorontsov, Egor, ...
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show more...
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Petranovic Niels ...
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Siewers, Verena, ...
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Engqvist, Martin ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Other Medical an ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
- Articles in the publication
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Data in Brief
- By the university
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University of Gothenburg
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Chalmers University of Technology