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Altered mRNA Expres...
Altered mRNA Expression Due to Rectal Perforation in a Porcine Model-A Pilot Study
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- Oikonomakis, Ioannis, 1977- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Surgery
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- Brodin, D. (author)
- Karolinska Institutet
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- Hörer, Tal M., 1971- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Cardiothoracic and Vascular Surgery
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- Skoog, Per (author)
- Örebro universitet,Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine,Institutionen för hälsovetenskaper,Department of Vascular Surgery and Institute of Medicine Department of Molecular and Clinical Medicine, Sahlgrenska University Hospital and Academy, Gothenburg, Sweden
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- Seilitz, Jenny, 1978- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Cardiothoracic and Vascular Surgery
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- Nilsson, Kristofer F., 1981- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Cardiothoracic and Vascular Surgery
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- Meehan, Adrian David, 1973- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Geriatrics
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- Jansson, Kjell, 1958- (author)
- Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Surgery
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(creator_code:org_t)
- 2022-05-31
- 2022
- English.
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In: Anticancer Research. - : Anticancer Research USA Inc.. - 0250-7005 .- 1791-7530. ; 42:6, s. 2827-2833
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https://doi.org/10.2...
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Abstract
Subject headings
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- Background: Anastomotic leakage is the most serious and unwelcome complication in rectal surgery. It has a great impact on postoperative morbidity and mortality. In this pilot study, changes of mRNA expression in blood were analyzed in an animal model designed to imitate anastomotic leakage. Materials and Methods: Twelve pigs were randomized into two groups: A sham-operated control group and an experimental group in which iatrogenic rectal perforation was performed. The changes in the mRNA expression at 4 hours after creating the perforation were studied. Microarray analysis was performed using Gene Chip whole porcine genome array. mRNA expression of 19,124 genes was investigated. Results: Significantly increased levels of genes with a fold change greater than 2 were found, including 276 coding for unknown proteins and 48 coding for known proteins. Eleven of those which coded for known proteins were up-regulated with a fold change >4. Conclusion: Eleven known genes were highly up-regulated after rectal perforation. These genes were mainly involved in inflammatory response, intracellular signaling and cell membrane regulation. Their corresponding proteins might potentially be clinical biomarkers of anastomotic leakage and should be evaluated in further clinical studies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Keyword
- Colorectal surgery
- anastomotic leakage
- gene expression analysis
- microarray
- low anterior resection
- anastomotic leakage
- intraperitoneal
- microdialysis
- diagnosis
- cytokines
- ischemia
- cancer
- Oncology
- Colorectal surgery
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Oikonomakis, Ioa ...
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Brodin, D.
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Hörer, Tal M., 1 ...
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Skoog, Per
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Seilitz, Jenny, ...
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Nilsson, Kristof ...
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show more...
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Meehan, Adrian D ...
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Jansson, Kjell, ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Surgery
- Articles in the publication
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Anticancer Resea ...
- By the university
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University of Gothenburg
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Örebro University
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Karolinska Institutet