Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo

Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo. Reactive oxygen species (ROS) have important roles in health and disease, but are chemically complex and difficult to measure accurately. This consensus statement proposes guidelines and best practices on the nomenclature and assessment of ROS, oxidative reactions and oxidative damage in cells, tissues and in vivo.

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Endokrinologi och diabetes hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Endocrinology and Diabetes hsv//eng
hydrogen-peroxide production
lipid-peroxidation
urinary
f-2-isoprostanes
fluorescent-probes
mass-spectrometry
superoxide
anion
redox regulation
quantification
oxidant
marker
Endocrinology & Metabolism

Bayir, H. (author)
Belousov, V. (author)
Chang, C. J. (author)
Davies, K. J. A. (author)
Davies, M. J. (author)
Dick, T. P. (author)
Finkel, T. (author)
Forman, H. J. (author)
Janssen-Heininger, Y. (author)
Gems, D. (author)
Kagan, V. E. (author)
Kalyanaraman, B. (author)
Larsson, N. G.Karolinska Institutet (author)
Milne, G. L. (author)
Nyström, Thomas,1960Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine(Swepub:gu)xnysth (author)
Poulsen, H. E. (author)
Radi, R. (author)
Van Remmen, H. (author)
Schumacker, P. T. (author)
Thornalley, P. J. (author)
Toyokuni, S. (author)
Winterbourn, C. C. (author)
Yin, H. Y. (author)
Halliwell, B. (author)
Karolinska InstitutetInstitutionen för biomedicin (creator_code:org_t)

In:Nature Metabolism: Springer Science and Business Media LLC4:6, s. 651-6622522-5812

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Endocrinology an ...

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