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Conjugative transfe...
Conjugative transfer of multi-drug resistance IncN plasmids from environmental waterborne bacteria to Escherichia coli
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- Jutkina, Jekaterina (author)
- Gothenburg University,Göteborgs universitet,CARe - Centrum för antibiotikaresistensforskning,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Centre for antibiotic resistance research, CARe,Institute of Biomedicine, Department of Infectious Medicine
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- Joffre, E. (author)
- Karolinska Institutet
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Agramont, J. (author)
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Mamani, N. (author)
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- Jutkina, Jekaterina (author)
- Gothenburg University,Göteborgs universitet,CARe - Centrum för antibiotikaresistensforskning,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Centre for antibiotic resistance research, CARe,Institute of Biomedicine, Department of Infectious Medicine
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- Boulund, F. (author)
- Karolinska Institutet
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Hu, Y. O. O. (author)
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- Jumilla-Lorenz, Daphne, 1997 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Farewell, Anne, 1961 (author)
- Gothenburg University,Göteborgs universitet,CARe - Centrum för antibiotikaresistensforskning,Institutionen för kemi och molekylärbiologi,Centre for antibiotic resistance research, CARe,Department of Chemistry and Molecular Biology
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- Larsson, D. G. Joakim, 1969 (author)
- Gothenburg University,Göteborgs universitet,CARe - Centrum för antibiotikaresistensforskning,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Centre for antibiotic resistance research, CARe,Institute of Biomedicine, Department of Infectious Medicine
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- Flach, Carl-Fredrik, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,CARe - Centrum för antibiotikaresistensforskning,Institute of Biomedicine, Department of Infectious Medicine,Centre for antibiotic resistance research, CARe
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Iniguez, V. (author)
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- Sjöling, Åsa, 1968 (author)
- Karolinska Institutet,Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
- 2022-10-26
- 2022
- English.
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In: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 13
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Abstract
Subject headings
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- Watersheds contaminated with municipal, hospital, and agricultural residues are recognized as reservoirs for bacteria carrying antibiotic resistance genes (ARGs). The objective of this study was to determine the potential of environmental bacterial communities from the highly contaminated La Paz River basin in Bolivia to transfer ARGs to an Escherichia coli lab strain used as the recipient. Additionally, we tested ZnSO4 and CuSO4 at sub-inhibitory concentrations as stressors and analyzed transfer frequencies (TFs), diversity, richness, and acquired resistance profiles. The bacterial communities were collected from surface water in an urban site close to a hospital and near an agricultural area. High transfer potentials of a large set of resistance factors to E. coli were observed at both sites. Whole-genome sequencing revealed that putative plasmids belonging to the incompatibility group N (IncN, IncN2, and IncN3) were predominant among the transconjugants. All IncN variants were verified to be mobile by a second conjugation step. The plasmid backbones were similar to other IncN plasmids isolated worldwide and carried a wide range of ARGs extensively corroborated by phenotypic resistance patterns. Interestingly, all transconjugants also acquired the class 1 integron intl1, which is commonly known as a proxy for anthropogenic pollution. The addition of ZnSO4 and CuSO4 at sub-inhibitory concentrations did not affect the transfer rate. Metal resistance genes were absent from most transconjugants, suggesting a minor role, if any, of metals in the spread of multidrug-resistant plasmids at the investigated sites.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- waterborne bacteria
- horizontal gene transfer
- multi-drug resistance
- Escherichia coli
- IncN plasmid
- copper sulfate
- zinc sulfate
- conjugative plasmid transfer
- antibiotic-resistance
- horizontal transfer
- anthropogenic activities
- metal resistance
- heavy-metals
- genes
- contamination
- copper
- nanoparticles
- animals
- Microbiology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Jutkina, Jekater ...
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Joffre, E.
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Agramont, J.
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Mamani, N.
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Boulund, F.
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show more...
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Hu, Y. O. O.
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Jumilla-Lorenz, ...
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Farewell, Anne, ...
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Larsson, D. G. J ...
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Flach, Carl-Fred ...
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Iniguez, V.
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Sjöling, Åsa, 19 ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Frontiers in Mic ...
- By the university
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University of Gothenburg
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Karolinska Institutet