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Potential Biomarker...
Potential Biomarkers of Acute Ischemic Stroke Etiology Revealed by Mass Spectrometry-Based Proteomic Characterization of Formalin-Fixed Paraffin-Embedded Blood Clots
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Rossi, R. (author)
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Mereuta, O. M. (author)
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Barbachan e Silva, M. (author)
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Molina Gil, S. (author)
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Douglas, A. (author)
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Pandit, A. (author)
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Gilvarry, M. (author)
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McCarthy, R. (author)
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O'Connell, S. (author)
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Tierney, C. (author)
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Psychogios, K. (author)
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Tsivgoulis, G. (author)
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Szikora, I. (author)
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- Tatlisumak, Turgut (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience
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- Rentzos, Alexandros, 1979 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för radiologi,Institute of Clinical Sciences, Department of Radiology
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Thornton, J. (author)
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Ó Broin, P. (author)
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Doyle, K. M. (author)
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(creator_code:org_t)
- 2022-04-19
- 2022
- English.
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In: Frontiers in Neurology. - : Frontiers Media SA. - 1664-2295. ; 13
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https://doi.org/10.3...
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Abstract
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- Background and Aims: Besides the crucial role in the treatment of acute ischemic stroke (AIS), mechanical thrombectomy represents a unique opportunity for researchers to study the retrieved clots, with the possibility of unveiling biological patterns linked to stroke pathophysiology and etiology. We aimed to develop a shotgun proteomic approach to study and compare the proteome of formalin-fixed paraffin-embedded (FFPE) cardioembolic and large artery atherosclerotic (LAA) clots. Methods: We used 16 cardioembolic and 15 LAA FFPE thrombi from 31 AIS patients. The thrombus proteome was analyzed by label-free quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS). MaxQuant v1.5.2.8 and Perseus v.1.6.15.0 were used for bioinformatics analysis. Protein classes were identified using the PANTHER database and the STRING database was used to predict protein interactions. Results: We identified 1,581 protein groups as part of the AIS thrombus proteome. Fourteen significantly differentially abundant proteins across the two etiologies were identified. Four proteins involved in the ubiquitin-proteasome pathway, blood coagulation or plasminogen activating cascade were identified as significantly abundant in LAA clots. Ten proteins involved in the ubiquitin proteasome-pathway, cytoskeletal remodeling of platelets, platelet adhesion or blood coagulation were identified as significantly abundant in cardioembolic clots. Conclusion: Our results outlined a set of 14 proteins for a proof-of-principle characterization of cardioembolic and LAA FFPE clots, advancing the proteome profile of AIS human thrombi and understanding the pathophysiology of ischemic stroke.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Keyword
- biomarkers stroke etiology
- FFPE proteomics
- mass spectrometry proteomics
- stroke biomarkers
- thrombus proteome
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Rossi, R.
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Mereuta, O. M.
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Barbachan e Silv ...
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Molina Gil, S.
-
Douglas, A.
-
Pandit, A.
-
show more...
-
Gilvarry, M.
-
McCarthy, R.
-
O'Connell, S.
-
Tierney, C.
-
Psychogios, K.
-
Tsivgoulis, G.
-
Szikora, I.
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Tatlisumak, Turg ...
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Rentzos, Alexand ...
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Thornton, J.
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Ó Broin, P.
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Doyle, K. M.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
- Articles in the publication
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Frontiers in Neu ...
- By the university
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University of Gothenburg