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Chimpanzee and pig-...
Chimpanzee and pig-tailed macaque iPSCs: Improved culture and generation of primate cross-species embryos
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Roodgar, M. (author)
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Suchy, F. P. (author)
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Nguyen, L. H. (author)
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Bajpai, V. K. (author)
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Sinha, R. (author)
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Vilches-Moure, J. G. (author)
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Van Bortle, K. (author)
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- Bhadury, Joydeep (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
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Metwally, A. (author)
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Jiang, L. H. (author)
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Jian, R. Q. (author)
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Chiang, R. (author)
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Oikonomopoulos, A. (author)
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Wu, J. C. (author)
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Weissman, I. L. (author)
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Mankowski, J. L. (author)
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Holmes, S. (author)
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Loh, K. M. (author)
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Nakauchi, H. (author)
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VandeVoort, C. A. (author)
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Snyder, M. P. (author)
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(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- English.
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In: Cell Reports. - : Elsevier BV. - 2211-1247. ; 40:9
- Related links:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- As our closest living relatives, non-human primates uniquely enable explorations of human health, disease, development, and evolution. Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. Here, we establish improved culture methods for chimpanzee (Pan troglodytes) and pig-tailed macaque (Macaca nemestrina) iPSCs. Such iPSCs sponta-neously differentiate in conventional culture conditions, but can be readily propagated by inhibiting endog-enous WNT signaling. As a unique functional test of these iPSCs, we injected them into the pre-implantation embryos of another non-human species, rhesus macaques (Macaca mulatta). Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre -implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- pluripotent stem-cells
- sendai-virus
- wnt
- derivation
- inhibition
- induction
- model
- superovulation
- oocytes
- target
- Cell Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Roodgar, M.
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Suchy, F. P.
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Nguyen, L. H.
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Bajpai, V. K.
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Sinha, R.
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Vilches-Moure, J ...
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show more...
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Van Bortle, K.
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Bhadury, Joydeep
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Metwally, A.
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Jiang, L. H.
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Jian, R. Q.
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Chiang, R.
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Oikonomopoulos, ...
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Wu, J. C.
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Weissman, I. L.
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Mankowski, J. L.
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Holmes, S.
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Loh, K. M.
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Nakauchi, H.
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VandeVoort, C. A ...
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Snyder, M. P.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Cell Reports
- By the university
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University of Gothenburg