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The PSI+ prion modu...
The PSI+ prion modulates cytochrome c oxidase deficiency caused by deletion of COX12
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Saini, P. K. (author)
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- Dawitz, Hannah, 1991- (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Aufschnaiter, Andreas (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Bondarev, S. (author)
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Thomas, J. (author)
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Amblard, A. (author)
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Stewart, J. (author)
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Thierry-Mieg, N. (author)
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- Ott, Martin, 1974 (author)
- Stockholms universitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Institutionen för biokemi och biofysik,University of Gothenburg, Sweden
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Pierrel, F. (author)
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(creator_code:org_t)
- 2022
- 2022
- English.
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In: Molecular Biology of the Cell. - 1059-1524 .- 1939-4586. ; 33:14
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Abstract
Subject headings
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- Cytochrome c oxidase (CcO) is a pivotal enzyme of the mitochondrial respiratory chain, which sustains bioenergetics of eukaryotic cells. Cox12, a peripheral subunit of CcO oxidase, is required for full activity of the enzyme, but its exact function is unknown. Here experimental evolution of a Saccharomyces cerevisiae.cox12 strain for similar to 300 generations allowed to restore the activity of CcO oxidase. In one population, the enhanced bioenergetics was caused by a A375V mutation in the cytosolic AAA+ disaggregase Hsp104. Deletion or overexpression of HSP104 also increased respiration of the Delta cox12 ancestor strain. This beneficial effect of Hsp104 was related to the loss of the [PSI+] prion, which forms cytosolic amyloid aggregates of the Sup35 protein. Overall, our data demonstrate that cytosolic aggregation of a prion impairs the mitochondrial metabolism of cells defective for Cox12. These findings identify a new functional connection between cytosolic proteostasis and biogenesis of the mitochondrial respiratory chain.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- yeast hsp104 chaperone
- saccharomyces-cerevisiae
- in-vivo
- mitochondrial
- translation
- propagation
- termination
- mutations
- evolution
- genes
- Cell Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Saini, P. K.
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Dawitz, Hannah, ...
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Aufschnaiter, An ...
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Bondarev, S.
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Thomas, J.
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Amblard, A.
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show more...
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Stewart, J.
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Thierry-Mieg, N.
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Ott, Martin, 197 ...
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Pierrel, F.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Molecular Biolog ...
- By the university
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University of Gothenburg
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Stockholm University