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Multiple traces of ...
Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia
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Wannigama, D. L. (author)
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Amarasiri, M. (author)
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Hongsing, P. (author)
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Hurst, C. (author)
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Modchang, C. (author)
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Chadsuthi, S. (author)
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Anupong, S. (author)
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- Phattharapornjaroen, Phatthranit (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery
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Rad, Smah (author)
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Fernandez, S. (author)
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Huang, A. T. (author)
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Kueakulpattana, N. (author)
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Tanasatitchai, C. (author)
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Vatanaprasan, P. (author)
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Saethang, T. (author)
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Luk-in, S. (author)
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Storer, R. J. (author)
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Ounjai, P. (author)
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Ragupathi, N. K. D. (author)
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Kanthawee, P. (author)
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Sano, D. (author)
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Furukawa, T. (author)
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Sei, K. (author)
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Leelahavanichkul, A. (author)
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Kanjanabuch, T. (author)
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Hirankarn, N. (author)
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Higgins, P. G. (author)
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Kicic, A. (author)
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Chatsuwan, T. (author)
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McLellan, A. D. (author)
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Abe, S. (author)
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(creator_code:org_t)
- Elsevier BV, 2023
- 2023
- English.
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In: SCIENCE OF THE TOTAL ENVIRONMENT. - : Elsevier BV. - 0048-9697. ; 858
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https://doi.org/10.1...
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Abstract
Subject headings
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- The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in waste-water from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.
Subject headings
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Miljövetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Environmental Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Ekologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Ecology (hsv//eng)
Keyword
- Monkeypox
- WBE
- Monkeypox viral DNA
- Wastewater surveillance
- Monkeypox
- virus in wastewater
- Monkeypox Asia
- Environmental Sciences & Ecology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Wannigama, D. L.
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Amarasiri, M.
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Hongsing, P.
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Hurst, C.
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Modchang, C.
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Chadsuthi, S.
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show more...
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Anupong, S.
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Phattharapornjar ...
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Rad, Smah
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Fernandez, S.
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Huang, A. T.
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Kueakulpattana, ...
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Tanasatitchai, C ...
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Vatanaprasan, P.
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Saethang, T.
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Luk-in, S.
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Storer, R. J.
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Ounjai, P.
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Ragupathi, N. K. ...
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Kanthawee, P.
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Sano, D.
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Furukawa, T.
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Sei, K.
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Leelahavanichkul ...
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Kanjanabuch, T.
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Hirankarn, N.
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Higgins, P. G.
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Kicic, A.
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Chatsuwan, T.
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McLellan, A. D.
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Abe, S.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Earth and Relate ...
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and Environmental Sc ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Ecology
- Articles in the publication
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SCIENCE OF THE T ...
- By the university
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University of Gothenburg