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The salivary proteo...
The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach
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van Leeuwen, S. J. M. (author)
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Proctor, G. B. (author)
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Staes, A. (author)
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Laheij, Amga (author)
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Potting, C. M. J. (author)
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Brennan, M. T. (author)
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- von Bültzingslöwen, Inger, 1947 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology
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Rozema, F. R. (author)
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Hazenberg, M. D. (author)
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Blijlevens, N. M. A. (author)
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Raber-Durlacher, J. E. (author)
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Huysmans, Mcdnjm (author)
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: Bmc Oral Health. - 1472-6831. ; 23:1
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
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- BackgroundOral mucositis is a frequently seen complication in the first weeks after hematopoietic stem cell transplantation recipients which can severely affects patients quality of life. In this study, a labelled and label-free proteomics approach were used to identify differences between the salivary proteomes of autologous hematopoietic stem cell transplantation (ASCT) recipients developing ulcerative oral mucositis (ULC-OM; WHO score & GE; 2) or not (NON-OM).MethodsIn the TMT-labelled analysis we pooled saliva samples from 5 ULC-OM patients at each of 5 timepoints: baseline, 1, 2, 3 weeks and 3 months after ASCT and compared these with pooled samples from 5 NON-OM patients. For the label-free analysis we analyzed saliva samples from 9 ULC-OM and 10 NON-OM patients at 6 different timepoints (including 12 months after ASCT) with Data-Independent Acquisition (DIA). As spectral library, all samples were grouped (ULC-OM vs NON-OM) and analyzed with Data Dependent Analysis (DDA). PCA plots and a volcano plot were generated in RStudio and differently regulated proteins were analyzed using GO analysis with g:Profiler.ResultsA different clustering of ULC-OM pools was found at baseline, weeks 2 and 3 after ASCT with TMT-labelled analysis. Using label-free analysis, week 1-3 samples clustered distinctly from the other timepoints. Unique and up-regulated proteins in the NON-OM group (DDA analysis) were involved in immune system-related processes, while those proteins in the ULC-OM group were intracellular proteins indicating cell lysis.ConclusionsThe salivary proteome in ASCT recipients has a tissue protective or tissue-damage signature, that corresponded with the absence or presence of ulcerative oral mucositis, respectively.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Keyword
- Oral mucositis
- Saliva
- TMT-labelled proteomics
- Label-free
- quantification
- Autologous hematopoietic stem cell transplantation
- Multiple myeloma
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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van Leeuwen, S. ...
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Proctor, G. B.
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Staes, A.
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Laheij, Amga
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Potting, C. M. J ...
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Brennan, M. T.
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show more...
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von Bültzingslöw ...
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Rozema, F. R.
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Hazenberg, M. D.
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Blijlevens, N. M ...
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Raber-Durlacher, ...
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Huysmans, Mcdnjm
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Dentistry
- Articles in the publication
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Bmc Oral Health
- By the university
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University of Gothenburg