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  • van Leeuwen, S. J. M. (author)

The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach

  • Article/chapterEnglish2023

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  • 2023

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  • LIBRIS-ID:oai:gup.ub.gu.se/329177
  • https://gup.ub.gu.se/publication/329177URI
  • https://doi.org/10.1186/s12903-023-03190-wDOI

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  • Language:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

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  • BackgroundOral mucositis is a frequently seen complication in the first weeks after hematopoietic stem cell transplantation recipients which can severely affects patients quality of life. In this study, a labelled and label-free proteomics approach were used to identify differences between the salivary proteomes of autologous hematopoietic stem cell transplantation (ASCT) recipients developing ulcerative oral mucositis (ULC-OM; WHO score & GE; 2) or not (NON-OM).MethodsIn the TMT-labelled analysis we pooled saliva samples from 5 ULC-OM patients at each of 5 timepoints: baseline, 1, 2, 3 weeks and 3 months after ASCT and compared these with pooled samples from 5 NON-OM patients. For the label-free analysis we analyzed saliva samples from 9 ULC-OM and 10 NON-OM patients at 6 different timepoints (including 12 months after ASCT) with Data-Independent Acquisition (DIA). As spectral library, all samples were grouped (ULC-OM vs NON-OM) and analyzed with Data Dependent Analysis (DDA). PCA plots and a volcano plot were generated in RStudio and differently regulated proteins were analyzed using GO analysis with g:Profiler.ResultsA different clustering of ULC-OM pools was found at baseline, weeks 2 and 3 after ASCT with TMT-labelled analysis. Using label-free analysis, week 1-3 samples clustered distinctly from the other timepoints. Unique and up-regulated proteins in the NON-OM group (DDA analysis) were involved in immune system-related processes, while those proteins in the ULC-OM group were intracellular proteins indicating cell lysis.ConclusionsThe salivary proteome in ASCT recipients has a tissue protective or tissue-damage signature, that corresponded with the absence or presence of ulcerative oral mucositis, respectively.

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  • Proctor, G. B. (author)
  • Staes, A. (author)
  • Laheij, Amga (author)
  • Potting, C. M. J. (author)
  • Brennan, M. T. (author)
  • von Bültzingslöwen, Inger,1947Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology(Swepub:gu)xvonin (author)
  • Rozema, F. R. (author)
  • Hazenberg, M. D. (author)
  • Blijlevens, N. M. A. (author)
  • Raber-Durlacher, J. E. (author)
  • Huysmans, Mcdnjm (author)
  • Göteborgs universitetInstitutionen för odontologi (creator_code:org_t)

Related titles

  • In:Bmc Oral Health23:11472-6831

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