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Mapping the Human C...
Mapping the Human Chondroitin Sulfate Glycoproteome Reveals an Unexpected Correlation Between Glycan Sulfation and Attachment Site Characteristics. : Chondroitin Sulfation and Attachment Site Characteristics
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- Noborn, Fredrik (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine,Univ Gothenburg, Inst Biomed, Dept Lab Med, Gothenburg, Sweden.
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- Nilsson, Jonas, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Core Facilities, Proteomics,Department of Laboratory Medicine,Core Facilities, Proteomics,Univ Gothenburg, Sahlgrenska Acad, Prote Core Facil, Gothenburg, Sweden.
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- Sihlbom, Carina, 1973 (author)
- Gothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics,Univ Gothenburg, Sahlgrenska Acad, Prote Core Facil, Gothenburg, Sweden.
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- Nikpour, Mahnaz, 1980 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine,Univ Gothenburg, Inst Biomed, Dept Lab Med, Gothenburg, Sweden.
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- Kjellén, Lena (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Larson, Göran, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine,Univ Gothenburg, Inst Biomed, Dept Lab Med, Gothenburg, Sweden.;Sahlgrens Univ Hosp, Lab Clin Chem, Gothenburg, Sweden.
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(creator_code:org_t)
- Elsevier, 2023
- 2023
- English.
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In: Molecular & cellular proteomics : MCP. - : Elsevier. - 1535-9484 .- 1535-9476. ; 22:8
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Abstract
Subject headings
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- Chondroitin sulfate proteoglycans (CSPGs) control key events in human health and disease and are composed of chondroitin sulfate (CS) polysaccharide(s) attached to different core proteins. Detailed information on the biological effects of site-specific CS structures is scarce as the polysaccharides are typically released from their core proteins prior to analysis. Here we present a novel glycoproteomic approach for site-specific sequencing of CS modifications from human urine. Software-assisted and manual analysis revealed that certain core proteins carried CS with abundant sulfate modifications, while others carried CS with lower levels of sulfation. Inspection of the amino acid sequences surrounding the attachment sites indicated that the acidity of the attachment site motifs increased the levels of CS sulfation, and statistical analysis confirmed this relationship. However, not only the acidity but also the sequence and characteristics of specific amino acids in the proximity of the serine glycosylation site correlated with the degree of sulfation. These results demonstrate attachment site-specific characteristics of CS polysaccharides of CSPGs in human urine and indicate that this novel method may assist in elucidating the biosynthesis and functional roles of CSPGs in cellular physiology.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Human Urine
- Chondroitin Sulfate Proteoglycans
- Metabolism
- Polysaccharides
- Amino Acid Sequence
- glycosaminoglycans
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Noborn, Fredrik
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Nilsson, Jonas, ...
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Sihlbom, Carina, ...
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Nikpour, Mahnaz, ...
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Kjellén, Lena
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Larson, Göran, 1 ...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Clinical Laborat ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Other Basic Medi ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Molecular & cell ...
- By the university
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University of Gothenburg
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Uppsala University