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Whole exome sequenc...
Whole exome sequencing of patients with varicella-zoster virus and herpes simplex virus induced acute retinal necrosis reveals rare disease-associated genetic variants
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Heinz, J. L. (author)
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Swagemakers, S. M. A. (author)
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- von Hofsten, Joanna, 1976 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience
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Helleberg, M. (author)
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Thomsen, M. M. (author)
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De Keukeleere, K. (author)
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de Boer, J. H. (author)
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Ilginis, T. (author)
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Verjans, Gmgm (author)
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van Hagen, P. M. (author)
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van der Spek, P. J. (author)
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Mogensen, T. H. (author)
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: Frontiers in Molecular Neuroscience. - 1662-5099. ; 16
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Abstract
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- Purpose: Herpes simplex virus (HSV) and varicella-zoster virus (VZV) are neurotropic human alphaherpesviruses endemic worldwide. Upon primary infection, both viruses establish lifelong latency in neurons and reactivate intermittently to cause a variety of mild to severe diseases. Acute retinal necrosis (ARN) is a rare, sight-threatening eye disease induced by ocular VZV or HSV infection. The virus and host factors involved in ARN pathogenesis remain incompletely described. We hypothesize an underlying genetic defect in at least part of ARN cases.Methods: We collected blood from 17 patients with HSV-or VZV-induced ARN, isolated DNA and performed Whole Exome Sequencing by Illumina followed by analysis in Varseq with criteria of CADD score > 15 and frequency in GnomAD < 0.1% combined with biological filters. Gene modifications relative to healthy control genomes were filtered according to high quality and read-depth, low frequency, high deleteriousness predictions and biological relevance.Results: We identified a total of 50 potentially disease-causing genetic variants, including missense, frameshift and splice site variants and on in-frame deletion in 16 of the 17 patients. The vast majority of these genes are involved in innate immunity, followed by adaptive immunity, autophagy, and apoptosis; in several instances variants within a given gene or pathway was identified in several patients.Discussion: We propose that the identified variants may contribute to insufficient viral control and increased necrosis ocular disease presentation in the patients and serve as a knowledge base and starting point for the development of improved diagnostic, prophylactic, and therapeutic applications.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- acute retinal necrosis (ARN)
- interferon
- autophagy
- apoptosis
- whole exome sequencing
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Heinz, J. L.
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Swagemakers, S. ...
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von Hofsten, Joa ...
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Helleberg, M.
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Thomsen, M. M.
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De Keukeleere, K ...
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show more...
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de Boer, J. H.
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Ilginis, T.
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Verjans, Gmgm
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van Hagen, P. M.
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van der Spek, P. ...
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Mogensen, T. H.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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Frontiers in Mol ...
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University of Gothenburg