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Genome-wide identif...
Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae
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Li, Qian (author)
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Feng, Peng (author)
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Tang, Hao (author)
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Lu, Fujia (author)
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Mou, Borui (author)
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Zhao, Lan (author)
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Li, Nan (author)
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Yang, Yaojun (author)
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Fu, Chun (author)
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Long, Wencong (author)
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Xiao, Ximeng (author)
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Li, Chaohao (author)
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Wu, Wei (author)
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Wang, Gang (author)
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- Liu, Beidong, 1972 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Tang, Tianle (author)
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Ma, Menggen (author)
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Wang, Hanyu (author)
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: BMC Microbiology. - 1471-2180. ; 23:1
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https://doi.org/10.1...
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Abstract
Subject headings
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- In bioethanol production, the main by-product, 5-hydroxymethylfurfural (HMF), significantly hinders microbial fermentation. Therefore, it is crucial to explore genes related to HMF tolerance in Saccharomyces cerevisiae for enhancing the tolerance of ethanol fermentation strains. A comprehensive analysis was conducted using genome-wide deletion library scanning and SGAtools, resulting in the identification of 294 genes associated with HMF tolerance in S. cerevisiae. Further KEGG and GO enrichment analysis revealed the involvement of genes OCA1 and SIW14 in the protein phosphorylation pathway, underscoring their role in HMF tolerance. Spot test validation and subcellular structure observation demonstrated that, following a 3-h treatment with 60mM HMF, the SIW14 gene knockout strain exhibited a 12.68% increase in cells with abnormal endoplasmic reticulum (ER) and a 22.41% increase in the accumulation of reactive oxygen species compared to the BY4741 strain. These findings indicate that the SIW14 gene contributes to the protection of the ER structure within the cell and facilitates the clearance of reactive oxygen species, thereby confirming its significance as a key gene for HMF tolerance in S. cerevisiae.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- 5-Hydroxymethylfurfural
- Saccharomyces cerevisiae
- SIW14
- Tolerance
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Li, Qian
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Feng, Peng
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Tang, Hao
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Lu, Fujia
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Mou, Borui
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Zhao, Lan
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show more...
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Li, Nan
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Yang, Yaojun
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Fu, Chun
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Long, Wencong
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Xiao, Ximeng
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Li, Chaohao
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Wu, Wei
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Wang, Gang
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Liu, Beidong, 19 ...
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Tang, Tianle
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Ma, Menggen
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Wang, Hanyu
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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BMC Microbiology
- By the university
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University of Gothenburg