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A Type II-B Cas9 nu...
A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo
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Bestas, Burcu (author)
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- Wimberger, Sandra, 1987 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Degtev, Dmitrii (author)
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Madsen, Alexandra (author)
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Rottner, Antje K. (author)
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Karlsson, Fredrik (author)
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Naumenko, Sergey (author)
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Callahan, Megan (author)
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Touza, Julia Liz (author)
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Francescatto, Margherita (author)
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Moller, Carl Ivar (author)
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Badertscher, Lukas (author)
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Li, Songyuan (author)
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Cerboni, Silvia (author)
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Selfjord, Niklas (author)
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Ericson, Elke (author)
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Gordon, Euan (author)
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Firth, Mike (author)
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Chylinski, Krzysztof (author)
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Taheri-Ghahfarokhi, Amir (author)
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Bohlooly-Y, Mohammad (author)
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Snowden, Mike (author)
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Pangalos, Menelaos (author)
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Nuttall, Barrett (author)
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Akcakaya, Pinar (author)
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Sienski, Grzegorz (author)
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Maresca, Marcello (author)
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: NATURE COMMUNICATIONS. - 2041-1723. ; 14:1
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Abstract
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- Streptococcus pyogenes Cas9 (SpCas9) and derived enzymes are widely used as genome editors, but their promiscuous nuclease activity often induces undesired mutations and chromosomal rearrangements. Several strategies for mapping off-target effects have emerged, but they suffer from limited sensitivity. To increase the detection sensitivity, we develop an off-target assessment workflow that uses Duplex Sequencing. The strategy increases sensitivity by one order of magnitude, identifying previously unknown SpCas9's off-target mutations in the humanized PCSK9 mouse model. To reduce off-target risks, we perform a bioinformatic search and identify a high-fidelity Cas9 variant of the II-B subfamily from Parasutterella secunda (PsCas9). PsCas9 shows improved specificity as compared to SpCas9 across multiple tested sites, both in vitro and in vivo, including the PCSK9 site. In the future, while PsCas9 will offer an alternative to SpCas9 for research and clinical use, the Duplex Sequencing workflow will enable a more sensitive assessment of Cas9 editing outcomes. SpCas9 unintended editing is a major concern. Here the authors report an off-target method using Duplex Sequencing with increased sensitivity for Cas9 mutation detection; they also identify a Cas9 variant of the II-B subfamily with intrinsic high fidelity (PsCas9) and see improved specificity.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Bestas, Burcu
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Wimberger, Sandr ...
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Degtev, Dmitrii
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Madsen, Alexandr ...
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Rottner, Antje K ...
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Karlsson, Fredri ...
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show more...
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Naumenko, Sergey
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Callahan, Megan
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Touza, Julia Liz
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Francescatto, Ma ...
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Moller, Carl Iva ...
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Badertscher, Luk ...
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Li, Songyuan
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Cerboni, Silvia
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Selfjord, Niklas
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Ericson, Elke
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Gordon, Euan
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Firth, Mike
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Chylinski, Krzys ...
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Taheri-Ghahfarok ...
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Bohlooly-Y, Moha ...
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Snowden, Mike
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Pangalos, Menela ...
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Nuttall, Barrett
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Akcakaya, Pinar
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Sienski, Grzegor ...
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Maresca, Marcell ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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NATURE COMMUNICA ...
- By the university
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University of Gothenburg